Correlation of CD34+ Cells with Tissue Angiogenesis after Traumatic Brain Injury in a Rat Model
Correlation of CD34+ Cells with Tissue Angiogenesis after Traumatic Brain Injury in a Rat Model
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DOI：
10.1089/neu.2008.0733
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发表时间：
2009-08-01
影响因子：
4.2
通讯作者：
Zhang, Jianning
中科院分区：
文献类型：
--
作者：
Guo, Xinbin;Liu, Li;Zhang, Jianning
越来越多的证据表明，循环内皮祖细胞（它是造血祖细胞CD34(+)细胞的一个亚群）在新生血管形成和组织修复中起着关键作用。我们在一个创伤性脑损伤（TBI）大鼠模型中验证了这样一个假设：创伤性脑损伤能够将CD34(+)细胞动员到外周血和脑组织中，这是血管修复的一个关键过程。雄性Wistar大鼠接受了可控性液压冲击。在创伤性脑损伤前后采集血液和脑组织，以测量外周血中CD34(+)细胞的水平，并检测它们在受损脑组织中的聚集情况。与手术对照组相比，创伤性脑损伤大鼠外周血中CD34(+)细胞显著增加，并在脑组织中聚集。免疫组织化学检测到具有不完全CD34(+)内皮样细胞衬里的新生血管，以及在损伤组织及其周围组织中微血管数量增加。结果表明，创伤性损伤后循环CD34(+)细胞的增加与创伤性脑损伤大鼠大脑中的血管生成密切相关。它们还表明，CD34(+)细胞移植或内源性CD34(+)细胞的增加可能是创伤性脑损伤患者的一种新的治疗方法。
Increasing evidence suggests that circulating endothelial progenitor cells, which are a subpopulation of hematopoietic progenitor CD34(+) cells, play a critical role in neovascularization and tissue repair. We have tested the hypothesis that traumatic brain injury (TBI) could mobilize CD34(+) cells to peripheral blood and brain tissue, a process critical for vascular repair, in a rat model of TBI. Male Wistar rats were subjected to controlled fluid percussion. Blood and brain tissue were collected before and after TBI to measure the levels of CD34(+) cells in peripheral blood and to detect their accumulation in the damaged cerebral tissue. Compared with surgery controls, CD34(+) cells significantly increased in the peripheral blood and accumulated in the brain tissue of TBI rats. Immunohistochemistry detected new vessels with incomplete CD34(+) endothelial-like cell lining and an increased number of microvessels in the injured and surrounding tissue. The results demonstrate a close correlation between an increase in circulating CD34(+) cells in response to traumatic injury and angiogenesis in TBI rat brain. They also suggest that transplantation of CD34(+) cells or augmentation of endogenous CD34(+) cells may be a novel therapeutic approach for patients with TBI.