Diversity in translational regulation.
Diversity in translational regulation.
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DOI：
10.1016/s0955-0674(00)00215-5
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发表时间：
2001-06
影响因子：
7.5
通讯作者：
P. Macdonald
中科院分区：
文献类型：
--
作者：
P. Macdonald
单个mRNA的翻译控制依赖于顺式调控元件，这些元件通常存在于3′非翻译区。这些调节细胞质多聚腺苷酸化的最佳特征，现在可以根据分子相互作用，蛋白质修饰及其后果来定义这个过程。生物化学和遗传学的方法已经推进了对果蝇身体模式至关重要的翻译调控的许多实例的理解。例如，体外翻译系统已被用于研究调控机制，遗传相互作用有助于建立调控因子和翻译装置组分之间的联系。虽然大多数控制的例子被认为是影响翻译的起始，两类调节因子，一个是蛋白质，一个是短的非编码RNA，现在似乎在延伸过程中抑制蛋白质合成。多样性似乎是翻译控制的核心特征，无论是在机制本身还是在使用这种形式的调节的情况下。
Translational control of individual mRNAs relies on cis-regulatory elements, which are often found in the 3′ untranslated region. The best characterized of these regulate cytoplasmic polyadenylation, and much of this process can now be defined in terms of molecular interactions, protein modifications and their consequences. Biochemical and genetic approaches have advanced the understanding of the many instances of translational regulation that are crucial for body patterning in Drosophila. For example, in vitro translation systems have been used to study the regulatory mechanisms, and genetic interactions have been instrumental in establishing a link between a regulatory factor and a component of the translational apparatus. Although most examples of control are thought to affect the initiation of translation, two classes of regulatory factors, one a protein and one a short non-coding RNA now appear to inhibit protein synthesis during elongation. Diversity seems to be a central feature of translational control, both in the mechanisms themselves and in the situations where this form of regulation is used.