In vivo release of catecholamines from xenogeneic chromaffin cell grafts with antidepressive activity.
In vivo release of catecholamines from xenogeneic chromaffin cell grafts with antidepressive activity.
复制标题
异种嗜铬细胞移植物体内释放具有抗抑郁活性的儿茶酚胺。
DOI：
10.1006/exnr.1994.1178
复制
发表时间：
1994
影响因子：
5.3
通讯作者：
Sagen,J
中科院分区：
文献类型：
--
作者：
Sortwell,CE;Petty,F;Kramer,G;Sagen,J
Previous studies in our laboratory have demonstrated that allografts of adrenal medullary tissue and xenografts of isolated bovine chromaffin cells to the rat frontal cortex can increase antidepressive activity in two separate animal models. Biochemical and pharmacological evidence suggest that the most likely mechanism of these antidepressive effects is via local release of catecholamines into the surrounding cortical parenchyma. The aim of the present study was to directly characterize the antidepressive mechanism of chromaffin cell xenografts by utilizingin vivomicrodialysis to measure extracellular catecholamine levels from bovine chromaffin cell and control implanted rat frontal cortex. Following transplantation, only bovine chromaffin cell grafted rats displayed significant increases in antidepressive activity, as assessed by the forced swimming test, compared to rats with grafts of bovine adrenal medullary fibroblasts or nontransplanted rats.In vivomicrodialysis results revealed remarkably elevated levels of epinephrine (EPI) and norepinephrine (NE), but not dopamine, in dialysates from bovine chromaffin cell-transplanted frontal cortex. The most likely source of these enhanced EPI and NE levels is the grafted xenogeneic chromaffin cells. The results of this study directly demonstrate that xenografts of bovine chromaffin cells to the rat frontal cortex provide a releasable pool of catecholamines for antidepressive activity.
