Metabolomics study of the hepatoprotective effect of total flavonoids of Mallotus apelta leaf in carbon tetrachloride-induced liver fibrosis in rats.
Metabolomics study of the hepatoprotective effect of total flavonoids of Mallotus apelta leaf in carbon tetrachloride-induced liver fibrosis in rats.
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DOI：
10.1002/bmc.5711
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发表时间：
2023-08
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影响因子：
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通讯作者：
Y. Ye;Bo Zhang;Wanting Mai;Yanjun Tan;Zhongwen Feng;Qiujie Huang
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文献类型：
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作者：
Y. Ye;Bo Zhang;Wanting Mai;Yanjun Tan;Zhongwen Feng;Qiujie Huang
广西壮族自治区《瑶药材质量标准》中记载的野桐叶，常用于治疗肝病。苋菜叶总黄酮(TFM)具有良好的抗纤维化活性，但TFM的抗纤维化机制尚不清楚。采用核磁共振技术研究TFM治疗前后尿液代谢物在CCl4诱导的肝纤维化中的动态变化。采用Ingenuity Path Analysis（IPA）寻找TFM改善肝纤维化的潜在靶基因，并通过实时荧光定量PCR和Western blotting验证靶基因的表达。 TFM可显着降低血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）水平，改善肝脏脂肪变性，减轻炎症；尿液代谢组学中，共发现7个潜在生物标志物，主要涉及两条代谢途径； IPA分析表明TNF可能是TFM改善CCl4诱导的大鼠肝纤维化的潜在靶点。本研究发现TNF可能是TFM治疗肝纤维化的潜在靶基因，并表明TFM的抗纤维化机制可以通过调节三羧酸循环和亚牛磺酸代谢来改善肝纤维化。
Mallotus apelta leaf, recorded in the quality standard of Yao Medicinal Material in Guangxi Zhuang autonomous region, is commonly used in the treatment of liver diseases. Total flavonoids of M. apelta leaf (TFM) had good anti-fibrosis activity, but the anti-fibrosis mechanism of TFM is still unclear. Nuclear magnetic resonance technology was used to study the dynamic changes of urine metabolites in CCl4 -induced liver fibrosis before and after TFM treatment. Ingenuity Path Analysis (IPA) was used to find potential target genes for TFM to improve liver fibrosis and verify the expression of target genes by real-time fluorescent quantitative PCR and Western blotting. TFM can significantly reduce serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) levels, improve liver steatosis and reduce inflammation; in urine metabolomics, a total of seven potential biomarkers were found, mainly involving two metabolic pathways; IPA analysis showed that TNF may be a potential target for TFM to improve liver fibrosis induced by CCl4 in rats. This study found that TNF may be a potential target gene for TFM treatment of liver fibrosis, and shows that the anti-fibrosis mechanism of TFM could improve liver fibrosis by regulating the tricarboxylic acid cycle and subtaurine metabolism.