Perchloric acid interference in enzymatic-fluorimetric-continuous-flow assay methods for measuring glucose, lactate, pyruvate, alanine, glycerol, and 3-hydroxybutyrate in blood.
Perchloric acid interference in enzymatic-fluorimetric-continuous-flow assay methods for measuring glucose, lactate, pyruvate, alanine, glycerol, and 3-hydroxybutyrate in blood.
复制标题
高氯酸对测量血液中葡萄糖、乳酸、丙酮酸、丙氨酸、甘油和 3-羟基丁酸的酶荧光连续流测定方法的干扰。
DOI：
10.1093/clinchem/32.6.1023
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发表时间：
1986
影响因子：
9.3
通讯作者：
A. Skillen
中科院分区：
文献类型：
--
作者：
R. Stappenbeck;A. Hodson;A. Skillen
高氯酸通常用于在测量组织、血液和其他体液中的各种代谢物之前使样品中的蛋白质变性和沉淀。然而，高氯酸可能通过抑制所用的酶而干扰分析过程。我们已经确定了高氯酸对葡萄糖，乳酸，丙酮酸，丙氨酸，甘油和3-羟基丁酸在血液中的酶荧光连续流动测定的影响。当样品中的高氯酸浓度与参比标准品不同时，这些代谢物的表观浓度会出现净增加或减少-其中一些差异是由于高氯酸离子的浓度，另一些是由于酸提取物的pH值。结果表明，需要向高氯酸中加入固定量的血液，或者中和并除去高氯酸盐。
Perchloric acid is commonly used to denature and precipitate proteins in samples before various metabolites are measured in tissue, blood, and other body fluids. However, perchloric acid can interfere in the analytical process, possibly by inhibiting the enzymes used. We have determined the effects of perchloric acid on measurements of glucose, lactate, pyruvate, alanine, glycerol, and 3-hydroxybutyrate in blood by enzymatic-fluorimetric-continuous-flow assays. There was a net increase or decrease in the apparent concentration of some of these metabolites when the perchloric acid concentration in the samples differed from that of the reference standards-some of these differences were due to the concentration of perchlorate ion and some to the pH of the acid extracts. The results show the need either to add a fixed amount of blood to perchloric acid or to neutralize and remove the perchlorate.