Dissociative capture of hot (3-13 eV) electrons by polypeptide polycations: an efficient process accompanied by secondary fragmentation
Dissociative capture of hot (3-13 eV) electrons by polypeptide polycations: an efficient process accompanied by secondary fragmentation
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DOI：
10.1016/s0009-2614(02)00149-5
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发表时间：
2002-04-22
影响因子：
2.8
通讯作者：
Zubarev, RA
中科院分区：
文献类型：
--
作者：
Kjeldsen, F;Haselmann, KF;Zubarev, RA
除了已知的最大电子捕获解离（ECD）的气相多肽聚阳离子在零电子能量，一个广泛的局部最大值被发现约10 eV。这个最大值是由于电子捕获之前的电子激发，如小阳离子的解离重组。在新的热电子捕获解离（HECD）机制中，不仅N-C-α键如在ECD中那样被裂解，而且由于过量的能量还诱导二次碎裂。有益的是，这种片段化包括从亮氨酸大量丢失.CH（CH 3）（2）和从异亮氨酸残基大量丢失. CH 2CH 3至切割位点末端，这允许区分这两种异构残基。(C)2002 Elsevier Science B. V.保留所有权利。
Beside the known maximum of electron capture dissociation (ECD) of gas-phase polypeptide polycations at zero electron energy, a broad local maximum is found around 10 eV. This maximum is due to electronic excitation prior to electron capture, as in dissociative recombination of small cations. In the novel hot electron capture dissociation (HECD) regime, not only N-C-alpha bonds are cleaved as in ECD, but secondary fragmentation is also induced due to the excess energy. Beneficially, this fragmentation includes abundant losses of .CH(CH3)(2) from leucine and .CH2CH3 from isoleucine residues terminal to the cleavage site, which allows for distinguishing between these two isomeric residues. (C) 2002 Elsevier Science B.V. All rights reserved.