Depth distribution of active bacteria and bacterial activity in lake sediment
Depth distribution of active bacteria and bacterial activity in lake sediment
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DOI：
10.1016/s0168-6496(03)00190-9
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发表时间：
2003-10-03
影响因子：
4.2
通讯作者：
Tranvik, L
中科院分区：
文献类型：
--
作者：
Haglund, AL;Lantz, P;Tranvik, L
沉积物中的细菌活性通常较低，因为细菌丰度普遍较高。尽管如此，即使在深层沉积物中也发现了很大一部分活跃的细菌。这些发现表明，沉积物细菌具有相对较低的细胞特异性生产。研究了湖泊沉积物中细菌的活性及其活性组分。通过胸苷和亮氨酸掺入和微量热法测定细菌的生产和代谢。除了总细菌计数外，我们还通过在DAPI染色方法中添加脱色步骤来估计细菌的含类核苷酸部分，并使用Live/Dead Baclight细菌活力试剂盒估计活部分。细菌的活性和丰度随沉积物深度的增加而降低，而活性细菌的比例在所有深度保持相似。57%至63%的细菌被评分为存活，13-52%被评分为含类核细胞。因此，在较深的沉积物中没有死细菌细胞的积累。如果在计数过程中不考虑沉积物细菌群落的活性部分，则沉积物细菌的细胞特异性生产可能被严重低估。(C)2003年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
The bacterial activity in sediments is often low considering the generally high bacterial abundance. Still, a large fraction of bacteria have been found active even in deep sediments. These findings suggest that sediment bacteria have comparatively low cell-specific production. We studied bacterial activity and the active fraction of bacteria in a lake sediment profile. Bacterial production and metabolism were measured by thymidine and leucine incorporation and by microcalorimetry. In addition to counts of total bacteria, we estimated the nucleoid-containing fraction of the bacteria by adding a destaining step to the DAPI staining method, and the live fraction using the Live/Dead Baclight bacterial viability kit. The bacterial activity and abundance decreased with sediment depth, while the proportion of active bacteria remained similar at all depths. Between 57 and 63% of the bacteria were scored viable, and 13-52% were scored as nucleoid-containing cells. Consequently, there was no accumulation of dead bacterial cells in deeper sediments. Cell-specific production of sediment bacteria may be severely underestimated if the active fraction of the sediment bacterial community is not considered during enumeration. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.