Transcriptional control of the dihydroflavonol 4-reductase multigene family in Lotus japonicus
Transcriptional control of the dihydroflavonol 4-reductase multigene family in Lotus japonicus
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DOI：
10.1007/s10265-010-0325-6
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发表时间：
2010-03
影响因子：
2.8
通讯作者：
Kazuko Yoshida;Rieko Iwasaka;N. Shimada;S. Ayabe;T. Aoki;M. Sakuta
中科院分区：
文献类型：
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作者：
Kazuko Yoshida;Rieko Iwasaka;N. Shimada;S. Ayabe;T. Aoki;M. Sakuta
在模式豆科植物百脉根的基因组中，二氢黄酮醇4-还原酶（dihydroflavonol 4-reductase，DFR）是花青素和原花青素（proanthocyanidin，PA）途径的第一个关键酶，由一个串联的五基因家族组成。表达分析显示，器官特异性和应激反应性在DFRs之间存在差异。为了阐明DFR表达的调控机制，我们研究了DFR多基因家族每个成员的转录调控。转录因子MYB、bHLH和WDR组合的异位表达表明，只有DFR 2启动子被激活，表明DFR 2基因家族的每个成员都是独立调节的。
In the genome of the model legumeLotus japonicus, dihydroflavonol 4-reductase (DFR), which is the first committed enzyme of the anthocyanin and proanthocyanidin (PA) pathways, is encoded as a tandemly arrayed five-gene family. Expression analysis revealed that both organ specificity and stress responsiveness differ among theDFRs. To elucidate the regulatory mechanisms underlying the expression ofDFRs, we investigated the transcriptional control of each member of theDFRmultigene family. Ectopic expression of a combination of the transcription factors MYB, bHLH, and WDR showed that only theDFR2promoter was activated, indicating that each member of theDFRgene family is regulated independently.