Activity of essential oils from Brazilian medicinal plants on Escherichia coli
Activity of essential oils from Brazilian medicinal plants on Escherichia coli
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DOI：
10.1016/j.jep.2006.11.034
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发表时间：
2007-05-04
影响因子：
5.4
通讯作者：
Sartoratto, Adilson
中科院分区：
文献类型：
--
作者：
Cristina Teixeira Duarte, Marta;Eduardo Leme, Ewerton;Sartoratto, Adilson
从巴西常用的 29 种药用植物的叶子中提取的精油针对 13 种不同的大肠杆菌血清型进行了筛选。使用 Clevenger 型系统通过水蒸馏获得油，并通过微量稀释法测定其最低抑菌浓度 (MIC)。马提尼香茅精油表现出广泛的抑制谱，对 13 种大肠杆菌血清型中的 10 种具有很强的活性（MIC 为 100 至 500 μg/mL）：三种产肠毒素、两种致病性、三种肠侵袭性和两种志贺毒素产生菌。 C. Winterianus 强烈抑制两种产肠毒素、一种肠病原、一种肠侵袭性和一种志贺毒素产生血清型。三叶木香还显示出良好的杀死大肠杆菌的潜力，具有中度至强的抑制作用。其他精油显示出抗菌特性，但针对所研究的血清型的作用更有限。通过气相色谱（GC）和气相色谱/质谱（GC-MS）对香茅精油进行化学分析，结果表明存在具有已知抗菌活性的化合物，包括香叶醇、乙酸香叶酯和反式石竹烯，单独测试表明香叶醇是抗菌活性化合物。马提尼香茅油的显着抗菌活性表明它们可以作为具有治疗潜力的化合物的来源。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
Essential oils obtained from leaves of 29 medicinal plants commonly used in Brazil were screened against 13 different Escherichia coli serotypes. The oils were obtained by water-distillation using a Clevenger-type system and their minimal inhibitory concentration (MIC) were determined by microdilution method. Essential oil from Cymbopogon martinii exhibited a broad inhibition spectrum, presenting strong activity (MIC between 100 and 500 mu g/mL) agains 10 out of 13 Escherichia coli serotypes: three enterotoxigenic, two enteropathogenic, three enteroinvasive and two shiga-toxin producers. C. winterianus inhibited strongly two enterotoxigenic, one enteropathogenic, one enteroinvasive and one shiga-toxin producer serotypes. Aloysia triphylla also shows good potential to kill Escherichia coli with moderate to strong inhibition. Other essential oils showed antimicrobial propertied, however with a more restricted action against the serotypes studied. Chemical analysis of Cymbopogon martinii essential oil performed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS) showed the presence of compounds with known antimicrobial activity, including geraniol, geranyl acetate and trans-cariophyllene, which tested separately, indicated geraniol as antimicrobial active compound. The significant antibacterial activity of Cymbopogon martinii oil suggests that they could serve as a source for compounds with therapeutic potential. (c) 2006 Elsevier Ireland Ltd. All rights reserved.