Cell culture chip using low-shear mass transport
Cell culture chip using low-shear mass transport
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DOI：
10.1021/la8003917
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发表时间：
2008-06-03
期刊：
影响因子：
3.9
通讯作者：
Pappas, Dimitri
中科院分区：
文献类型：
--
作者：
Liu, Ke;Pitchimani, Rajasekar;Pappas, Dimitri
我们已经开发了一种流动池，其允许在稳定、均匀和低剪切力的环境中培养贴壁细胞以及悬浮细胞。该装置具有连续介质供应和废物交换功能。在本文中，一个简单而快速的协议，用于设备的设计，制造和组装（密封）的基础上的聚（二甲基硅氧烷）（PMDS）/载玻片混合结构进行了描述。监测细胞培养系统性能，并测定培养孔内的有效剪切力。通过操纵装置尺寸和体积流速，在实验期间控制剪切应力。通过显微镜观察长期培养期间的细胞粘附、生长、增殖和死亡。内皮细胞和悬浮细胞在该装置中的生长表现出与传统方法相当的特性。低剪切培养装置显著降低了微流体系统中遇到的剪切应力，允许粘附和悬浮细胞在简单装置中生长。
We have developed a flow cell that allows culturing adherent cells as well as suspended cells in a stable, homogeneous, and low-shear force environment. The device features continuous medium supply and waste exchange. In this paper, a simple and fast protocol for device design, fabrication, and assembly (sealing) based on a poly(dimethylsiloxane) (PMDS)/glass slide hybrid structure is described. The cell culture system performance was monitored, and the effective shear force inside the culture well was also determined. By manipulating the device dimensions and volumetric flow rate, shear stress was controlled during experiments. Cell adhesion, growth, proliferation, and death over long-term culture periods were observed by microscopy. The growth of both endothelial and suspension cells in this device exhibited comparable characteristics to those of traditional approaches. The low-shear culture device significantly reduced shear stress encountered in microfluidic systems, allowing both adherent and suspended cells to be grown in a simple device.