Overlay analysis of the oligonucleotide array gene expression profiles and copy number abnormalities as determined by array comparative genomic hybridization in medulloblastomas
Overlay analysis of the oligonucleotide array gene expression profiles and copy number abnormalities as determined by array comparative genomic hybridization in medulloblastomas
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DOI：
10.1002/gcc.20388
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发表时间：
2007-01-01
影响因子：
3.7
通讯作者：
Cowell, John K.
中科院分区：
文献类型：
--
作者：
Lo, Ken C.;Rossi, Michael R.;Cowell, John K.
基因表达阵列数据和基于阵列的比较基因组杂交数据的联合分析已经在一系列26个儿童脑肿瘤中被使用，以确定与涉及特定染色体区域的丢失、获得和扩增相一致的上调和下调基因。染色体臂3q、6q、8p、10q、16q、17p频繁丢失，7号染色体、9p和17q染色体臂增加。只有一个肿瘤的2p区扩增，这与MYCN和DDX1基因的表达增加相对应。为了便于分析这两个数据集，我们开发了一个定制的覆盖工具，该工具定义了在整个基因组中，特别是在髓母细胞瘤复发的区域内，在缺失区域低表达和在获得区域高表达的基因。(C)2006年Wiley-Liss，Inc.
Combined analysis of gene expression array data and array-based comparative genomic hybridization data have been used in a series of 26 pediatric brain tumors to define up- and downregulated genes that coincide with losses, gains, and amplifications involving specific chromosome regions. Frequent losses were defined in chromosome arms 3q, 6q, 8p, 10q, 16q, 17p, and gains were identified in chromosome 7, and chromosome arms 9p and 17q. Amplification of a 2p region was seen in only one tumor, which corresponded to increased expression of the MYCN and DDX1 genes. To facilitate the analysis of the two data sets, we have developed a custom overlay tool that defines genes that are underexpressed in regions of deletions and overexpressed in regions of gain, across the genome and specifically within regions showing recurrent involvement in medulloblastomas. (c) 2006 Wiley-Liss, Inc.