Osmotin is a homolog of mammalian adiponectin and controls apoptosis in yeast through a homolog of mammalian adiponectin receptor
Osmotin is a homolog of mammalian adiponectin and controls apoptosis in yeast through a homolog of mammalian adiponectin receptor
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DOI：
10.1016/j.molcel.2004.11.050
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发表时间：
2005-01-21
期刊：
影响因子：
16
通讯作者：
Bressan, RA
中科院分区：
文献类型：
--
作者：
Narasimhan, ML;Coca, MA;Bressan, RA
植物防御蛋白PR-5家族的抗真菌活性一直被怀疑与真菌靶点的特定质膜成分有关。渗透蛋白是一种烟草PR-5家族蛋白，在酿酒酵母中诱导细胞凋亡。我们在这里发现，ORE20/PHO36 （YOL002c）编码的蛋白是一种渗透素结合的质膜蛋白，它是一种调节脂质和磷酸盐代谢的七跨膜结构域受体样多肽，是对渗透素完全敏感所必需的。PHO36在渗透素诱导的凋亡通路中位于RAS2的上游。PH036的哺乳动物同源物是激素脂联素的受体，调节细胞脂质和糖代谢。渗透素和脂联素是相应的“受体”结合蛋白，它们的序列并不相似。然而，这两种蛋白的P桶结构域可以重叠，渗透素和脂联素一样，通过脂联素受体激活C2C12肌细胞中的AMP激酶。
The antifungal activity of the PR-5 family of plant defense proteins has been suspected to involve specific plasma membrane component(s) of the fungal target. Osmotin is a tobacco PR-5 family protein that induces apoptosis in the yeast Saccharomyces cerevisiae. We show here that the protein encoded by ORE20/PHO36 (YOL002c), a seven transmembrane domain receptor-like polypeptide that regulates lipid and phosphate metabolism, is an osmotin binding plasma membrane protein that is required for full sensitivity to osmotin. PHO36 functions upstream of RAS2 in the osmotin-induced apoptotic pathway. The mammalian homolog of PH036 is a receptor for the hormone adiponectin and regulates cellular lipid and sugar metabolism. Osmotin and adiponectin, the corresponding "receptor" binding proteins, do not share sequence similarity. However, the P barrel domain of both proteins can be overlapped, and osmotin, like adiponectin, activates AMP kinase in C2C12 myocytes via adiponectin receptors.