Immune cross-reactivity in Celiac disease: Anti-gliadin antibodies bind to neuronal synapsin I
Immune cross-reactivity in Celiac disease: Anti-gliadin antibodies bind to neuronal synapsin I
复制标题
DOI：
10.4049/jimmunol.178.10.6590
复制
发表时间：
2007-05-15
影响因子：
4.4
通讯作者：
Latov, Norman
中科院分区：
文献类型：
--
作者：
Alaedini, Armin;Okamoto, Haruka;Latov, Norman
乳糜泻是一种免疫介导的疾病，由遗传易感个体摄入小麦醇溶蛋白和相关蛋白引发。除了特征性肠病外，乳糜泻还伴有各种肠外表现，包括神经系统并发症，如神经病变、共济失调、癫痫发作和神经行为改变。神经系统表现的原因尚不清楚，但已提出麦胶蛋白和神经系统蛋白之间的分子模拟引起的自身免疫起作用。在这项研究中，我们试图研究抗麦醇溶蛋白抗体对神经蛋白的免疫反应性。我们的特点是结合亲和纯化的抗麦胶蛋白抗体从免疫动物脑蛋白的一维和二维凝胶电泳，免疫印迹，肽质量映射。主要的免疫反应蛋白被鉴定为突触蛋白I。来自患有乳糜泻的患者的抗麦醇溶蛋白Ab也与蛋白质结合。这种交叉反应性可能提供线索的致病机制与面筋敏感性相关的神经功能缺损。免疫学杂志，2007，178：6590-6595.
Celiac disease is an immune-mediated disorder triggered by ingestion of wheat gliadin and related proteins in genetically susceptible individuals. In addition to the characteristic enteropathy, celiac disease is associated with various extraintestinal manifestations, including neurologic complications such as neuropathy, ataxia, seizures, and neurobehavioral changes. The cause of the neurologic manifestations is unknown, but autoimmunity resulting from molecular mimicry between gliadin and nervous system proteins has been proposed to play a role. In this study, we sought to investigate the immune reactivity of the anti-gliadin Ab response toward neural proteins. We characterized the binding of affinity-purified anti-gliadin Abs from immunized animals to brain proteins by one- and two-dimensional gel electrophoresis, immunoblotting, and peptide mass mapping. The major immunoreactive protein was identified as synapsin I. Anti-gliadin Abs from patients with celiac disease also bound to the protein. Such cross-reactivity may provide clues into the pathogenic mechanism of the neurologic deficits that are associated with gluten sensitivity. The Journal of Immunology, 2007, 178: 6590-6595.