CAPILLARY GAS-CHROMATOGRAPHIC PROFILING OF URINARY, PLASMA AND ERYTHROCYTE SUGARS AND POLYOLS AS THEIR TRIMETHYLSILYL DERIVATIVES, PRECEDED BY A SIMPLE AND RAPID PREPURIFICATION METHOD
CAPILLARY GAS-CHROMATOGRAPHIC PROFILING OF URINARY, PLASMA AND ERYTHROCYTE SUGARS AND POLYOLS AS THEIR TRIMETHYLSILYL DERIVATIVES, PRECEDED BY A SIMPLE AND RAPID PREPURIFICATION METHOD
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DOI：
10.1016/0009-8981(86)90303-7
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发表时间：
1986-06-30
影响因子：
5
通讯作者：
VANDERSLIK, W
中科院分区：
文献类型：
--
作者：
JANSEN, G;MUSKIET, FAJ;VANDERSLIK, W
描述了一种毛细管气相色谱分析尿液、血浆、未洗涤和洗涤红细胞中三甲基硅化单糖、双糖和多元醇的方法。该预纯化方法是基于在生理量的无机盐存在下对衍生化的适度抑制以及所形成的三甲基硅油醚对水和稀盐酸处理的相对稳定性。给出了汇集尿液中31种糖/多元醇的系列到系列质量控制数据。该方法用于建立72名自由饮食对照者尿液中18种糖/多元醇的年龄依赖性浓度。本文介绍并讨论了以下病例的尿的气相色谱分析和定量数据：采用低乳糖和半乳糖饮食治疗的半乳糖血症患儿，采用低苯丙氨酸和酪氨酸饮食治疗的1型遗传性酪氨酸血症患儿，以及采用高热量胃滴注喂养的神经系统疾病患儿。本文给出了一个健康成人的血浆、未洗涤和洗涤的红细胞以及治疗前患有半乳糖血症的新生儿的血浆中的糖/多元醇谱的例子。
A capillary gas chromatographic method for the profiling of trimethylsilylated mono- and disaccharides and polyols in urine, plasma and unwashed and washed erythrocytes is described. The prepurification method is based on the moderate inhibition of the derivatization experienced in the presence of physiological amounts of inorganic salts and the relative stability of the formed trimethylsilylethers towards treatment with water and dilute hydrochloride acid. Series to series quality control data for 31 sugars/polyols in a pooled urine are given. The method was used to establish age-dependent concentrations of 18 sugars/polyols in urines of 72 control persons on a free diet. Gas chromatographic profiles and quantitative data obtained from urines of pediatric patients with galactosemia treated with a diet low in lactose and galactose, type 1 hereditary tyrosinemia treated with a diet low in phenylalanine and tyrosine, and a neurological disorder with a high calory gastric drip feeding, are presented and discussed. Examples of the profiling of sugars/polyols in the plasma, unwashed and washed erythrocytes of a healthy adult, and the plasma of a newborn with galactosemia prior to treatment, are given.