miR-30b suppresses tumor migration and invasion by targeting EIF5A2 in gastric cancer
miR-30b suppresses tumor migration and invasion by targeting EIF5A2 in gastric cancer
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DOI：
10.3748/wjg.v21.i31.9337
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发表时间：
2015-08-21
影响因子：
4.3
通讯作者：
Yan, Chao
中科院分区：
文献类型：
--
作者：
Tian, Shu-Bo;Yu, Jian-Chun;Yan, Chao
目的：目的：探讨miR-30 b在胃癌中的生物学作用及其抑制胃癌细胞转移的分子机制。CCK-8法检测miR-30 b过表达对胃癌细胞增殖的影响。流式细胞术检测miR-30 b对细胞凋亡的影响。Transwell实验用于迁移和侵袭实验。结果：miR-30 b在胃癌组织和胃癌细胞系中表达下调，具有抑癌作用。过表达miR-30 b可促进胃癌细胞株AGS和MGC 803的凋亡，抑制其增殖、迁移和侵袭能力。生物信息学分析证实真核生物翻译起始因子5A 2（EIF 5A 2）的3 '非翻译区是miR-30 b的假定结合位点。Luci-ferase报告基因分析和Western blot分析证实EIF 5A 2基因是miR-30 b的靶基因。结论：miR-30 b模拟物转染后，EIF 5A 2靶基因E-cadherin和Vimentin的表达水平发生了变化，提示miR-30 b与EIF 5A 2之间存在着联系，EIF 5A 2在介导上皮-间充质转化中起着重要作用。
AIM: To elucidate the potential biological role of miR-30b in gastric cancer and investigate the underlying molecular mechanisms of miR-30b to inhibit metastasis of gastric cancer cells.METHODS: The expression of miR-30b was detected in gastric cancer cell lines and samples by reverse transcription-polymerase chain reaction. CCK-8 assays were conducted to explore the impact of miR-30b overexpression on the proliferation of gastric cancer cells. Flow cytometry was used to examine the effect of miR-30b on the apoptosis. Transwell test was used for the migration and invasion assays. Luciferase reporter assays and Western blot were employed to validate regulation of putative target of miR-30b.RESULTS: The results showed that miR-30b was downregulated in gastric cancer tissues and cancer cell lines and functioned as a tumor suppressor. Overexpression of miR-30b promoted cell apoptosis, and suppressed proliferation, migration and invasion of the gastric cancer cell lines AGS and MGC803. Bioinformatic analysis identified the 3'-untranslated region of eukaryotic translation initiation factor 5A2 (EIF5A2) as a putative binding site of miR-30b. Luci-ferase reporter assays and Western blot analysis confirmed the EIF5A2 gene as a target of miR-30b. Moreover, expression levels of the EIF5A2 targets E-cadherin and Vimentin were altered following trans-fection of miR-30b mimics.CONCLUSION: Our findings describe a link between miR-30b and EIF5A2, which plays an important role in mediating epithelial-mesenchymal transition.