Formation of Dentinal Bridge on Surface of Regenerated Dental Pulp in Dentin Defects by Controlled Release of Fibroblast Growth Factor-2 From Gelatin Hydrogels
Formation of Dentinal Bridge on Surface of Regenerated Dental Pulp in Dentin Defects by Controlled Release of Fibroblast Growth Factor-2 From Gelatin Hydrogels
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DOI：
10.1016/j.joen.2009.03.049
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发表时间：
2009-06-01
影响因子：
4.2
通讯作者：
Terashita, Masamichi
中科院分区：
文献类型：
--
作者：
Ishimatsu, Hirotaka;Kitamura, Chiaki;Terashita, Masamichi
引言：牙髓再生治疗是克服传统治疗诱导修复性牙本质形成的局限性的重要手段。在本研究中，我们研究了不同剂量的成纤维细胞生长因子-2（FGF-2）从明胶水凝胶中的控制释放对牙本质牙髓复合体再生的影响。研究方法：在切断大鼠磨牙的牙髓后，将含有不同剂量FGF-2的明胶水凝胶单独植入切断的牙髓部位上方的牙本质缺损中。观察离体牙髓上牙本质缺损区的组织学变化及牙本质基质蛋白-1（dentinmatrixprotein-1，dentin 1）和巢蛋白（nestin）的表达。结果如下：我们发现，高剂量的FGF-2从明胶水凝胶中的控制释放诱导增殖牙髓中的α-1阳性钙化颗粒，而中等剂量的FGF-2诱导增殖牙髓表面上的α-1阳性牙本质桥。这些发现表明释放的FGF-2的剂量对牙本质缺损中再生的钙化组织的结构有影响。结论：本实验结果表明，可生物降解明胶凝胶中FGF-2的加入量可以调控离断牙髓的牙本质再生，而不是对离断牙髓的修复性牙本质形成。（J Endod 2009;35：858-865）
Introduction: Pulp regeneration therapy is important to overcome the limitations of conventional therapy to induce reparative dentinogenesis. In the present study, we examined the effects of controlled release of different dosages of fibroblast growth factor-2 (FGF-2) from gelatin hydrogels to regenerate the dentin-pulp complex. Methods: After the amputation of dental pulp of rat molars, gelatin hydrogels incorporating various dosages of FGF-2 were individually implanted into dentin defects above the sites of the amputated pulps. Histologic changes as well as the expression of dentin matrix protein-1 (DMP-1) and nestin in the dentin defect area above the amputated pulp were analyzed. Results: We found that controlled release of high doses of FGF-2 from gelatin hydrogels induced DMP-1-positive calcified particles in the proliferating pulp, whereas a moderate dose of FGF-2 induced DMP-1-positive dentinal bridge on the surface of the proliferating pulp. These findings indicate that the dosage of released FGF-2 has an influence on the structure of calcified tissue regenerated in dentin defects. In addition, pulp cells near calcified tissues regenerated in dentin defects were nestin-negative, suggesting that the calcified tissues might be osteodentin, Conclusions: Our results showed that the dentin regeneration on amputated pulp, not reparative dentin formation toward amputated pulp, can be regulated by adjusting the dosage of FGF-2 incorporated in biodegradable gelatin hydrogels. (J Endod 2009;35:858-865)