Characterization of rat calcitonin mRNA.
Characterization of rat calcitonin mRNA.
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大鼠降钙素 mRNA 的表征。
DOI：
10.1073/pnas.77.8.4444
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发表时间：
1980
影响因子：
11.1
通讯作者：
Evans,RM
中科院分区：
文献类型：
--
作者：
Amara,SG;David,DN;Rosenfeld,MG;Roos,BA;Evans,RM
构建了与大鼠降钙素mRNA互补的cDNA嵌合质粒。部分序列分析表明，该插入物含有一个编码降钙素完整氨基酸序列的核苷酸序列。两个基本氨基酸在激素序列之前，三个基本氨基酸在激素序列之后，这表明降钙素是由较大前体的蛋白质水解裂解产生的，其方式类似于其他小多肽激素。已知在分泌激素中被修饰的cooh末端脯氨酸，在前体中被甘氨酸跟随。克隆的降钙素DNA用于表征降钙素mRNA的表达。产降钙素的大鼠甲状腺髓样癌细胞质mrna和正常大鼠甲状腺细胞质mrna含有一个单一物种，长1050个核苷酸，与克隆的降钙素cDNA杂交。在产生大量免疫反应性降钙素的肿瘤中，降钙素mRNA序列的浓度更高。来自其他内分泌组织的rna，包括大鼠垂体前叶和神经中间叶，不含可检测到的降钙素mRNA。
A chimeric plasmic containing cDNA complementary to rat calcitonin mRNA has been constructed. Partial sequence analysis shows that the insert contains a nucleotide sequence encoding the complete amino acid sequence of calcitonin. Two basic amino acids precede and three basic amino acids follow the hormone sequence, suggesting that calcitonin is generated by the proteolytic cleavage of a larger precursor in a manner analogous to that of other small polypeptide hormones. The COOH-terminal proline, known to be amidated in the secreted hormone, is followed by a glycine in the precursor. The cloned calcitonin DNA was used to characterize the expression of calcitonin mRNA. Cytoplasmic mRNAs from calcitonin-producing rat medullary thyroid carcinoma lines and from normal rat thyroid glands contain a single species, 1050 nucleotides long, whch hybridizes to the cloned calcitonin cDNA. The concentration of calcitonin mRNA sequences is greater in those tumors that produce larger amounts of immunoreactive calcitonin. RNAs from other endocrine tissues, including anterior and neurointermediate lobes of rat pituitary, contain no detectable calcitonin mRNA.