Targeting P2X7 receptor inhibits the metastasis of murine P388D1 lymphoid neoplasm cells to lymph nodes
Targeting P2X7 receptor inhibits the metastasis of murine P388D1 lymphoid neoplasm cells to lymph nodes
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DOI：
10.1042/cbi20090428
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发表时间：
2010-12-01
影响因子：
3.9
通讯作者：
Zuo, Yunfei
中科院分区：
文献类型：
--
作者：
Ren, Shuangyi;Zhang, Yi;Zuo, Yunfei
P2X(7)R （P2X(7)受体）是正常细胞中表达的atp门控阳离子通道，参与细胞增殖和凋亡。在这里，我们证实了P2X(7)R在小鼠P388D1淋巴样肿瘤细胞中的表达。此外，发现atp刺激的P2X(7)R表达可触发细胞内钙通量增加。此外，短发夹RNA沉默和P2X7R抗体阻断可显著降低P388D1细胞向淋巴结的转移。这些结果表明，抑制P2X7R的表达和功能可减弱小鼠淋巴样肿瘤细胞系P388D1的转移能力，为抗转移治疗提供了新的潜在靶点。
The P2X(7)R (P2X(7) receptor) is an ATP-gated cation channel expressed in normal cells that participates in both cell proliferation and apoptosis. Here, we have confirmed P2X(7)R expression on murine P388D1 lymphoid neoplasm cells. In addition, ATP-stimulated P2X(7)R expression was found to trigger increased intracellular calcium flux. Furthermore, silencing with short hairpin RNA and blocking with P2X7R antibody significantly reduced the metastasis of P388D1 cells to lymph nodes. These results indicate that inhibition of the expression and function of P2X7R attenuates the metastatic ability of murine lymphoid neoplasm cell line P388D1, which represents a new potential target for anti-metastatic therapy.