Increased fatty acid production in potato by engineering of acetyl-CoA carboxylase
Increased fatty acid production in potato by engineering of acetyl-CoA carboxylase
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DOI：
10.1007/s00425-004-1236-3
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发表时间：
2004-07-01
期刊：
影响因子：
4.3
通讯作者：
Dörmann, P
中科院分区：
文献类型：
--
作者：
Klaus, D;Ohlrogge, JB;Dörmann, P
马铃薯（Solanum tuberosum L.）其特征在于块茎中储存了大量淀粉。为了评估马铃薯块茎中油合成的能力，在碳水化合物或脂质代谢改变的转基因马铃薯中探索脂质含量和进入脂质合成的通量的变化。在ADP-葡萄糖焦磷酸化酶或质体磷酸葡萄糖变位酶的反义系中观察到的淀粉量的强烈下降对储存脂质含量没有影响。类似地，过表达拟南芥（Arabidopsis thaliana（L.）嘿含有增加量的淀粉的质体ATP/ADP转运体的油含量没有改变，表明质体ATP水平不限制马铃薯块茎中的脂肪酸合成。然而，过表达的乙酰辅酶A羧化酶从拟南芥中的淀粉体的马铃薯块茎导致脂肪酸合成的增加和三酰甘油的量增加了5倍以上。总之，这些数据表明，马铃薯块茎具有储存脂质合成的能力，丙二酰辅酶A，脂肪酸合成过程中延长的底物，是油积累的限制因素之一。
In contrast to oil seeds, potato (Solanum tuberosum L.) is characterized by a high amount of starch stored in the tubers. To assess the capacity for oil synthesis in potato tubers, the changes in lipid content and flux into lipid synthesis were explored in transgenic potatoes altered in carbohydrate or lipid metabolism. A strong decrease in the amount of starch observed in antisense lines for ADP-glucose pyrophosphorylase or plastidic phosphoglucomutase had no effect on storage-lipid content. Similarly, potato lines over-expressing the Arabidopsis thaliana (L.) Heynh. plastidic ATP/ADP transporter that contained an increased amount of starch were not altered in oil content, indicating that the plastidic ATP level is not limiting fatty acid synthesis in potato tubers. However, over-expression of the acetyl-CoA carboxylase from Arabidopsis in the amyloplasts of potato tubers led to an increase in fatty acid synthesis and a more than 5-fold increase in the amount of triacylglycerol. Taken together, these data demonstrate that potato tubers have the capacity for storage-lipid synthesis and that malonyl-CoA, the substrate for elongation during fatty acid synthesis, represents one of the limiting factors for oil accumulation.