Expression and clinical significance of miR-181a and miR-203 in systemic lupus erythematosus patients.
Expression and clinical significance of miR-181a and miR-203 in systemic lupus erythematosus patients.
复制标题
DOI：
--
复制
发表时间：
2017-11
影响因子：
3.3
通讯作者：
H-S Li;Y. Ning;S-B Li;P. Shao;S-J Chen;Q. Ye;X. Heng
中科院分区：
文献类型：
--
作者：
H-S Li;Y. Ning;S-B Li;P. Shao;S-J Chen;Q. Ye;X. Heng
目的MiR-181 a在调节T细胞和B细胞分化以及免疫应答中起重要作用。其异常表达可能参与了系统性红斑狼疮（SLE）的发病机制。miR-203参与调节Toll样受体和诱导免疫耐受。miR-203的异常表达或功能与多种自身免疫性疾病有关，但其在SLE中的作用尚不清楚。因此，本研究通过检测血清中miR-181 a和miR-203的水平，分析其在SLE诊断和评估中的作用。患者与方法收集我院SLE患者，根据疾病活动指数分为非活动性和活动性SLE，沿着健康人。采用qRT-PCR定量检测血清中miR-181 a和miR-203的表达，并分析其与临床特征的相关性。采用ROC曲线评价其对SLE的诊断价值，并比较高、低表达人群的无进展生存期（PFS）。结果SLE患者血清中miR-181 a水平显著升高，miR-203水平显著降低，且均与SLE活动性相关。miR-181 a和miR-203的表达水平与红细胞沉降率、C反应蛋白、抗dsDNA抗体、补体和SLEDAI评分相关。它们的表达水平对活动期系统性红斑狼疮的鉴别诊断有一定价值（AUC=0.885和0.843）。miR-181 a高表达组的PFS低于miR-181低表达组（χ2=7.474，p=0.029）。miR-203高表达组患者的PFS显著高于低表达组（χ2=4.367，p=0.037）。结论SLE患者miR-181 a表达增高，miR-203表达降低，提示miR-181 a可能对SLE的诊断和病情评估具有重要意义。
OBJECTIVE MiR-181a plays a critical role in modulating T cell and B cell differentiation, as well as immune response. Its abnormal expression probably participates in the pathogenesis of systemic lupus erythematosus (SLE). MiR-203 is involved in regulating Toll-like receptor and inducing immune tolerance. Abnormal expression or function of miR-203 is related to multiple auto-immune diseases but its role in SLE remains unclear. This study, thus, investigated the serum level of miR-181a and miR-203, to analyze their roles in diagnosing and evaluating SLE. PATIENTS AND METHODS SLE patients were recruited from our hospital, and divided into non-active and active SLE based on disease activity index, along with healthy individuals. qRT-PCR was used to quantify the serum miR-181a and miR-203 expression, and their correlation with clinical features. ROC was used to evaluate the diagnostic value on SLE, while survival curves were compared to show progression-free survival (PFS) between populations with high and low expression. RESULTS SLE patients had significantly higher serum levels of miR-181a and lower miR-203, both of which were correlated with SLE activity. Expression levels of miR-181a and miR-203 were correlated with erythrocyte sedimentation rate, C reactive protein, anti-dsDNA antibody, complements, and SLEDAI score. Their expression levels had certain values in the differential diagnosis for active SLE (AUC=0.885 and 0.843). PFS in miR-181a high-expression individuals was lower than that in the low-miR-181 group (χ2=7.474, p=0.029). Whilst, miR-203 high-expression SLE patients had higher PFS than low-expression group (χ2=4.367, p=0.037). CONCLUSIONS SLE patients had higher miR-181a and lower miR-203 expression, which thus may have critical implications in disease diagnosis and evaluation.