Assessing the impacts of temperature and storage on Escherichia coli, Salmonella, and L. monocytogenes decay in dairy manure
Assessing the impacts of temperature and storage on Escherichia coli, Salmonella, and L. monocytogenes decay in dairy manure
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DOI：
10.1007/s00449-016-1569-x
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发表时间：
2016-06-01
影响因子：
3.8
通讯作者：
Farver, Thomas B.
中科院分区：
文献类型：
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作者：
Biswas, Sagor;Pandey, Pramod K.;Farver, Thomas B.
环境水中动物粪便传播的病原体水平升高是一个严重的人类健康问题。减轻奶牛粪便等动物粪便中的病原体流入土壤和水中，需要改进我们现有的关于奶牛粪便处理方法中减少病原体的知识。本研究旨在加深对乳化液粪便在厌氧（AN）和有限好氧（LA）贮存条件下人类病原体腐烂的认识。在用液体浆料饲喂的间歇反应器中，对三种病原体（大肠杆菌、沙门氏菌和单核增生李斯特菌）的腐烂进行了评估。我们测试了一系列温度条件（30、35、42和50℃），以确定温度对AN和LA条件下大肠杆菌、沙门氏菌和单核增生乳杆菌腐烂的影响。结果表明，与沙门氏菌和单核增生乳杆菌相比，大肠杆菌在LA和AN环境中的存活时间更长。病原菌在温度和环境条件下（即LA和AN）的存活率变化表明，有必要开发改进的奶牛粪便废物处理方法来控制动物粪便传播的病原体。本研究的结果将有助于提高目前对奶牛粪便中人类病原体腐烂的理解，以便利益相关者做出明智的动物粪便处理决策。
Elevated levels of animal waste-borne pathogen in ambient water is a serious human health issue. Mitigating influx of pathogens from animal waste such as dairy manure to soil and water requires improving our existing knowledge of pathogen reductions in dairy manure treatment methods. This study was conducted to enhance the understanding of human pathogen decay in liquid dairy manure in anaerobic (AN) and limited aerobic (LA) storage conditions. The decay of three pathogens (Escherichia coli, Salmonella spp., and Listeria monocytogenes) was assessed in bench-scale batch reactors fed with liquid slurry. A series of temperatures (30, 35, 42, and 50 degrees C) conditions were tested to determine the impacts of temperature on Escherichia coli, Salmonella, and L. monocytogenes decay in AN and LA conditions. Results showed prolonged survival of E. coli compared to Salmonella and L. monocytogenes in both LA and AN environments. Variations in survival among pathogens with temperature and environmental conditions (i.e., LA and AN) indicated the necessity of developing improved dairy manure waste treatment methods for controlling animal waste-borne pathogens. The results of this study will help in improving the current understanding of human pathogen decay in dairy manure for making informed decisions of animal manure treatment by stakeholders.