Nondestructive Techniques to Evaluate the Characteristics and Development of Engineered Cartilage.
Nondestructive Techniques to Evaluate the Characteristics and Development of Engineered Cartilage.
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DOI：
10.1007/s10439-015-1535-9
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发表时间：
2016-03
影响因子：
3.8
通讯作者：
Welter JF
中科院分区：
文献类型：
--
作者：
Mansour JM;Lee Z;Welter JF
本文就组织工程软骨的特性评价方法进行综述。其中许多已被开发用于评估天然和骨关节炎关节软骨的特性。然而，随着人们对工程化软骨的兴趣越来越大，需要专门的方法来对组织在发育过程中和植入后进行无损评价。需要这样的方法，部分是由于组织的细胞组分的性能的大的供体间和供体内可变性，这仍然是递送可靠的TE软骨用于植入的障碍。使用常规的破坏性测试，这种可变性使得几乎不可能在特定的工程化组织片的水平上预测组织工程化过程的时机和结果，并且还使得难以评估改变组织工程化方案的影响。虽然很明显，工程化软骨的真正测试是其植入后的性能，但在体外和/或体内非破坏性地确定的植入前和植入后性质与性能的相关性应导致预测方法，以改善质量控制并最大限度地减少植入劣质组织的机会。
In this review, methods for evaluating the properties of tissue engineered (TE) cartilage are described. Many of these have been developed for evaluating properties of native and osteoarthritic articular cartilage. However, with the increasing interest in engineering cartilage, specialized methods are needed for nondestructive evaluation of tissue while it is developing and after it is implanted. Such methods are needed, in part, due to the large inter- and intra-donor variability in the performance of the cellular component of the tissue, which remains a barrier to delivering reliable TE cartilage for implantation. Using conventional destructive tests, such variability makes it near-impossible to predict the timing and outcome of the tissue engineering process at the level of a specific piece of engineered tissue and also makes it difficult to assess the impact of changing tissue engineering regimens. While it is clear that the true test of engineered cartilage is its performance after it is implanted, correlation of pre and post implantation properties determined non-destructively in vitro and/or in vivo with performance should lead to predictive methods to improve quality-control and to minimize the chances of implanting inferior tissue.