Photoacoustic probes for real-time tracking of endogenous H(2)S in living mice.
Photoacoustic probes for real-time tracking of endogenous H(2)S in living mice.
复制标题
用于实时跟踪活体小鼠内源性 H2S 的光声探针。
DOI：
10.1039/c6sc04703c
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发表时间：
2017-03-01
期刊：
影响因子：
8.4
通讯作者：
Zhao C
中科院分区：
文献类型：
--
作者：
Shi B;Gu X;Fei Q;Zhao C
H2S激活探针显示出对H2S的极快和高度选择性的光声响应，允许在活小鼠中进行实时光声捕获。H2S是一种重要的化学介质，在多种生理和病理过程中发挥着重要作用。然而，由于缺乏可靠的分析方法，内源性H2S生成的体内跟踪仍然很困难。在这里，我们提出了第一个例子的可激活的光声探针实时成像的H2S在活的小鼠通过充分利用光声成像模式的优势，在精细的空间分辨率在深组织穿透。所设计的探针可以在H2S存在下在780 nm处产生高的近红外吸收，从而在近红外区域产生强的光声信号输出。此外，该探针具有非常快速和高选择性的响应性，良好的水溶性和优异的生物相容性。鉴于这些突出的特点，这种探针实现了直接光声捕获的H2S产生的HCT116荷瘤小鼠模型。这些初步的成像研究表明，HCT 116结肠肿瘤表现出CBS上调活性，导致H2S生成速率增加。
H2S-activatable probes showed an extremely fast and highly selective photoacoustic response to H2S, permitting real-time photoacoustic trapping in living mice. H2S is a key chemical mediator that exerts a vital role in diverse physiological and pathological processes. However, in vivo tracking of endogenous H2S generation still remains difficult due to the lack of reliable analytical methods. Herein, we present the first example of activatable photoacoustic probes for real-time imaging of H2S in living mice through the full utilization of the superiority of photoacoustic imaging modality at fine spatial resolution during deep tissue penetration. The designed probe can generate high NIR absorption at 780 nm in the presence of H2S, thus producing a strong photoacoustic signal output in the NIR region. Furthermore, this probe exhibits extremely fast and highly selective responsiveness, good water-solubility and excellent biocompatibility. In light of these outstanding features, this probe realizes the direct photoacoustic trapping of endogenous H2S generation in a HCT116 tumor-bearing mouse model. These preliminary imaging studies show that HCT116 colon tumors exhibit CBS upregulation activity, resulting in an increased rate of H2S generation.