Pulse stimulation with odors or IBMX/forskolin potentiates responses in isolated olfactory neurons.
Pulse stimulation with odors or IBMX/forskolin potentiates responses in isolated olfactory neurons.
复制标题
用气味或 IBMX/毛喉素进行脉冲刺激可增强孤立嗅觉神经元的反应。
DOI：
10.1093/chemse/bjj017
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Delay,RonaJ
中科院分区：
文献类型：
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作者：
Zhang,Wenling;Delay,RonaJ
许多气味反应是由腺苷3′，5 ′-环磷酸（cAMP）途径介导的，其中cAMP门控电流被Ca 2+依赖性Cl−电流放大。在嗅觉神经元中，长时间暴露于气味会降低气味反应，这是一种适应性效应。一些研究表明，气味适应与细胞内Ca 2+升高有关。在本研究中，使用穿孔配置的膜片钳技术，我们发现，重复的气味刺激eliferan增强随后的反应在嗅觉神经元。这种增强是模仿刺激cAMP途径，并没有出现相关的磷酸化的离子通道，因为蛋白激酶抑制剂不能阻断it. Our数据表明，局部增加[Ca 2 +]ivia激活cAMP途径介导的脉冲引起的增强。在第一个气味应用中，通过环核苷酸门控通道进入的Ca 2+似乎被缓冲。重复刺激允许局部增加[Ca 2 +]i，在每个后续气味脉冲中招募更多的Ca 2+依赖性Cl−通道。
Many odor responses are mediated by the adenosine 3′,5′-cyclic monophosphate (cAMP) pathway in which the cAMP-gated current is amplified by Ca2+-dependent Cl−current. In olfactory neurons, prolonged exposure to odors decreases the odor response and is an adaptive effect. Several studies suggest that odor adaptation is linked to elevated intracellular Ca2+. In the present study, using the perforated configuration of the patch clamp technique, we found that repetitive odor stimulation elicits a potentiation of the subsequent responses in olfactory neurons. This potentiation is mimicked by stimulating the cAMP pathway and does not appear to be related to phosphorylation of ion channels since protein kinase inhibitors could not block it. Our data suggest that local increases in [Ca2+]ivia activation of the cAMP pathway mediate the pulse-elicited potentiation. In the first odor application, entry of Ca2+through cyclic nucleotide–gated channels appears to be buffered. Repetitive stimulation allows local increases in [Ca2+]i, recruiting more Ca2+-dependent Cl−channels with each subsequent odor pulse.