FgCDC14 regulates cytokinesis, morphogenesis, and pathogenesis in Fusarium graminearum
FgCDC14 regulates cytokinesis, morphogenesis, and pathogenesis in Fusarium graminearum
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FgCDC14 调节禾谷镰刀菌的胞质分裂、形态发生和发病机制
DOI：
10.1111/mmi.13157
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发表时间：
2015-11-01
影响因子：
3.6
通讯作者：
Xu, Jin-Rong
中科院分区：
文献类型：
--
作者：
Li, Chaohui;Melesse, Michael;Xu, Jin-Rong
Cdc14磷酸酶的成员在动物和真菌中很常见，但在植物中不存在。虽然其同源物在植物病原真菌中保守，但其在侵染过程中的功能尚不清楚。在本研究中，我们发现CDC14同源基因在禾谷镰刀菌的发病和形态发生中起重要作用。FgCDC14是正常细胞分裂和隔膜形成所必需的，并且FgCDC14具有对Cdk型磷酸化位点子集特异性的磷酸酶活性。Fgcdc14突变体的生长、分生和子囊孢子形成减少。它在子囊孢子发生和发病机制上有缺陷。Fgcdc14的分裂减少，菌丝室含有多个细胞核，表明核分裂和细胞质分裂之间的协调存在缺陷。有趣的是，突变分生孢子的足细胞经常分化为分生细胞，从而产生相互连接的分生孢子。在间期，FgCdc14 - GFP定位于细胞核和纺锤体-极体。综上所述，我们的研究结果表明，Cdc14磷酸酶可能通过抵消Cdk磷酸化，在禾谷镰刀菌的细胞分裂和隔膜形成中起作用，并且是植物感染所必需的。
Members of Cdc14 phosphatases are common in animals and fungi, but absent in plants. Although its orthologs are conserved in plant pathogenic fungi, their functions during infection are not clear. In this study, we showed that the CDC14 ortholog is important for pathogenesis and morphogenesis in Fusarium graminearum. FgCDC14 is required for normal cell division and septum formation and FgCdc14 possesses phosphatase activity with specificity for a subset of Cdk‐type phosphorylation sites. The Fgcdc14 mutant was reduced in growth, conidiation, and ascospore formation. It was defective in ascosporogenesis and pathogenesis. Septation in Fgcdc14 was reduced and hyphal compartments contained multiple nuclei, indicating defects in the coordination between nuclear division and cytokinesis. Interestingly, foot cells of mutant conidia often differentiated into conidiogenous cells, resulting in the production of inter‐connected conidia. In the interphase, FgCdc14‐GFP localized to the nucleus and spindle‐pole‐body. Taken together, our results indicate that Cdc14 phosphatase functions in cell division and septum formation in F. graminearum, likely by counteracting Cdk phosphorylation, and is required for plant infection.