RADIX: rhizoslide platform allowing high throughput digital image analysis of root system expansion.
RADIX: rhizoslide platform allowing high throughput digital image analysis of root system expansion.
复制标题
DOI：
10.1186/s13007-016-0140-8
复制
发表时间：
2016
期刊：
影响因子：
5.1
通讯作者：
Hund A
中科院分区：
文献类型：
--
作者：
Le Marié C;Kirchgessner N;Flütsch P;Pfeifer J;Walter A;Hund A
根区中基因型与环境相互作用的表型对于作物改良具有重要意义，因为植物根系的空间分布对于植物获得土壤的水和养分资源至关重要。然而，到目前为止，它是不清楚的遗传变异在多大程度上根系响应空间变化的土壤资源可以用于育种应用。其中，一个限制因素是缺乏允许分析这种相互作用的表型分析平台。我们开发了一个系统，能够（a）同步监测根和芽的生长，（B）调查他们的动态响应和（c）分析的影响，异质性N分布的部分根系在分裂的营养设置与吞吐量（200个玉米植株一次）足以映射的数量性状基因座或多个环境因素的屏幕。在试验中，24个玉米基因型在分氮条件下生长，并研究了地上部和根系生长的反应。在高氮条件下，所有基因型的冠部和侧根伸长速率几乎都增加一倍。基因型特异性反应的强度变化很大。例如，生长最快和最慢的基因型之间冠根的伸长几乎相差两倍。一个更强的选择性的根放置在高氮室有关的增加拍摄的发展表明，早期的活力可能与更激烈的觅食行为。据我们所知，RADIX是目前存在的唯一系统，它允许研究冠根分裂营养应用的差异反应，以量化基因组定位或选择实验中的觅食行为。在这样做时，可以研究根和芽发育的变化以及与植物性能的关系。本文的在线版本（doi：10.1186/s13007-016-0140-8）包含补充材料，可供授权用户使用。
Phenotyping of genotype-by-environment interactions in the root-zone is of major importance for crop improvement as the spatial distribution of a plant’s root system is crucial for a plant to access water and nutrient resources of the soil. However, so far it is unclear to what extent genetic variations in root system responses to spatially varying soil resources can be utilized for breeding applications. Among others, one limiting factor is the absence of phenotyping platforms allowing the analysis of such interactions. We developed a system that is able to (a) monitor root and shoot growth synchronously, (b) investigate their dynamic responses and (c) analyse the effect of heterogeneous N distribution to parts of the root system in a split-nutrient setup with a throughput (200 individual maize plants at once) sufficient for mapping of quantitative trait loci or for screens of multiple environmental factors. In a test trial, 24 maize genotypes were grown under split nitrogen conditions and the response of shoot and root growth was investigated. An almost double elongation rate of crown and lateral roots was observed under high N for all genotypes. The intensity of genotype-specific responses varied strongly. For example, elongation of crown roots differed almost two times between the fastest and slowest growing genotype. A stronger selective root placement in the high-N compartment was related to an increased shoot development indicating that early vigour might be related to a more intense foraging behaviour. To our knowledge, RADIX is the only system currently existing which allows studying the differential response of crown roots to split-nutrient application to quantify foraging behaviour in genome mapping or selection experiments. In doing so, changes in root and shoot development and the connection to plant performance can be investigated. The online version of this article (doi:10.1186/s13007-016-0140-8) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
6.9
作者：
Adu MO;Chatot A;Wiesel L;Bennett MJ;Broadley MR;White PJ;Dupuy LX
通讯作者：
Dupuy LX
影响因子：
6.9
作者：
GRANATO, TC;RAPER, CD
通讯作者：
RAPER, CD
影响因子：
7.3
作者：
De Kroon, Hans;Visser, Eric J. W.;Hutchings, Michael J.
通讯作者：
Hutchings, Michael J.
DOI：
10.1071/bi9721169
发表时间：
1972-01-01
期刊：
AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES
影响因子：
--
作者：
HACKETT, C
通讯作者：
HACKETT, C
HTPHENO：高通量植物表型的图像分析管道。
影响因子：
3
作者：
Hartmann A;Czauderna T;Hoffmann R;Stein N;Schreiber F
通讯作者：
Schreiber F