Wide range of lineages of cells expressing nerve growth factor mRNA in the nerve lesions of patients with vasculitic neuropathy: An implication of endoneurial macrophage for nerve regeneration
Wide range of lineages of cells expressing nerve growth factor mRNA in the nerve lesions of patients with vasculitic neuropathy: An implication of endoneurial macrophage for nerve regeneration
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DOI：
10.1016/j.neuroscience.2004.06.083
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Sobue, G
中科院分区：
文献类型：
--
作者：
Mitsuma, N;Yamamoto, M;Sobue, G
为探讨神经生长因子(NGF)在血管性神经病患者神经病变中的定位作用。NGF mRNA原位杂交和免疫组织化学双重标记结果显示，NGF mRNA表达于雪旺细胞、浸润性巨噬细胞、T细胞和血管周围细胞等多种细胞系。具有早期特征的圆形巨噬细胞表达高水平的NGF mRNA，而晚期多态巨噬细胞表达低水平的NGF mRNA。具有多种细胞表面标志的T细胞也普遍表达NGF mRNA。损伤神经周围神经外膜巨噬细胞和神经内膜T细胞高水平表达NGF mRNA。此外，神经内神经NGF在巨噬细胞中的表达水平与轴突再生程度密切相关。这些结果表明，NGF在多种细胞系中都有表达，但在血管神经损伤中的表达存在空间差异，并且表达的NGF，尤其是巨噬细胞，可能在神经再生过程中发挥重要作用。(C)2004年IBRO。爱思唯尔有限公司出版。保留所有权利。
In situ localization of nerve growth factor (NGF) mRNA was examined in the nerve lesions of patients with vasculitic neuropathy. Double labeling of in situ hybridization for NGF mRNA and immunohistochemistry for cell markers showed that NGF mRNA was expressed in a wide range of lineages of cells: Schwann cells, infiltrating macrophages, T cells and perivascular cells. Round-shaped macrophages with early-phase features expressed high levels of NGF mRNA, in contrast to late-phase polymorphic macrophages, which expressed low levels of NGF mRNA. NGF mRNA was also expressed universally in T cells with various cell surface markers. Epineurial macrophages surrounding vasculitic lesions and endoneurial T cells expressed high levels of NGF mRNA in the damaged nerves. Moreover, the extent of endoneurial NGF expression level in macrophages was closely related to the degree of axonal regeneration. These results suggest that NGF is expressed in a wide range of lineages of cells but is differentially expressed spatially in vasculitic nerve lesions, and that the expressed NGF, particularly in macrophages, may play an important role in the nerve regeneration process. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.