Bimetallic AgAu decorated MWCNTs enable robust nonenzyme electrochemical sensors for in-situ quantification of dopamine and H2O2 biomarkers expelled from PC-12 cells
Bimetallic AgAu decorated MWCNTs enable robust nonenzyme electrochemical sensors for in-situ quantification of dopamine and H2O2 biomarkers expelled from PC-12 cells
复制标题
双金属 AgAu 修饰的 MWCNT 可实现强大的非酶电化学传感器，用于对 PC-12 细胞排出的多巴胺和 H2O2 生物标志物进行原位定量
DOI：
10.1016/j.jelechem.2020.114554
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发表时间：
2020-12-01
影响因子：
4.5
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Balasubramanian, Paramasivam;He, Shao-Bin;Chen, Wei
Construction of efficient and reliable biosensor for real-time quantification of neurochemicals and reactive oxygen species is highly essential for clinical analysis because of their close connection to the health of living beings, however, remains challenging. Here, we demonstrated a novel amperometric sensor based on tiny AgAu nanoparticles (similar to 2.15 nm) decorated multiwalled carbon nanotubes (MWCNTs) with boosted sensitivity and specificity for in-vivo monitoring of extracellular dopamine (DA) and hydrogen peroxide (H2O2). According to basic analysis, AgAu-MWCNTs nanohybrid electrode showed significantly higher electroactive surface area (0.259 cm(2)), and superior heterogeneous electron transfer rate constant (9.25 x 10(2) cm s(-1)). Profiting fromthe synergism and high conductivity of nanoparticular AgAu and MWCNTs, fabricated sensor delivered excellent electrochemical activity, can quantify DA and H2O2 with a broad dynamic response in the range of 3 nM-2.3 mM and 7 nM-7.8 mM, respectively. Also, the estimated limit of detection of DA and H2O2 to be 0.23 nM and 0.59 nM, correspondingly, which is well inside the desired level for in-situ monitoring. It was successfully applied to measure DA and H2O2 concentration expelled from the PC-12 cells upon the external stimulation, signifying a potential value of such biosensor in cell biology and clinical disease and cancer analysis.
