Active micro-mixers using surface acoustic waves on Y-cut 128° LiNbO3
Active micro-mixers using surface acoustic waves on Y-cut 128° LiNbO3
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DOI：
10.1088/0960-1317/16/3/009
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发表时间：
2006-03-01
影响因子：
2.3
通讯作者：
Lee, GB
中科院分区：
文献类型：
--
作者：
Tseng, WK;Lin, JL;Lee, GB
本研究提出一种利用表面声波（SAW）快速混合并流流体的微混合器的主动方法。由于微流体系统的低雷诺数流动条件，混合是具有挑战性的工作。SAW器件在128度Y切割的铌酸锂（LiNbO 3）上制造。微混合器是与聚二甲基硅氧烷微通道集成的这些压电致动器。实验研究了叉指换能器（IDT）上的外加电压以及平行和横向两种微混合器布局对混合性能的影响。实验结果表明，并联式混合器具有较高的混合效果。同时，较高的施加电压的叉指换能器导致的混合性能的有源微混合器的显着改善。还研究了与IDT上的施加电压相关的典型温度效应。最后，进行蛋白质（血红蛋白）和酶（胰蛋白酶）之间的消化反应，以验证微混合器的能力。使用质谱法对蛋白质-酶混合物进行定性分析。使用这些基于SAW的混合器，消化的肽的量增加。此外，还使用高效液相色谱法对蛋白质-酶混合物进行了定量分析。实验数据表明，使用主动混合器，消化肽的量增加21.1%。因此，所开发的微混合器可以应用于微流体系统中，以提高混合效率，从而增强生物反应。
This study presents an active method for micro-mixers using surface acoustic waves (SAW) to rapidly mix co-fluent fluids. Mixing is challenging work in microfluidic systems due to their low-Reynolds-number flow conditions. SAW devices were fabricated on 128 degrees Y-cut lithium niobate (LiNbO3). The micro-mixers; are these piezoelectric actuators integrated with polydimethylsiloxane microchannels. The effects of the applied voltages on interdigitated transducers (IDTs) and two layouts, parallel- and transversal-type, of micro-mixers on the mixing performance were experimentally explored. The experimental results revealed that the parallel-type mixer achieved a higher mixing effect. Meanwhile, a higher applied voltage on the IDTs led to a significant improvement in the mixing performance of the active micro-mixer. Typical temperature effects associated with the applied voltages on the IDTs were also investigated. Finally, a digestion reaction between a protein (hemoglobin) and an enzyme (trypsin) was performed to verify the capability of the micro-mixers. The protein-enzyme Mixture was qualitatively analyzed using mass spectrometry. Using these SAW-based mixers, the amount of digested peptides increased. Additionally, the protein-enzyme mixture was also quantitatively analyzed using high-performance liquid chromatography. Experimental data showed that the amount of digested peptides increased 21.1% using the active mixer. Therefore, the developed micro-mixers can be applied in microfluidic systems for improving mixing efficiency and thus enhancing the bio-reaction.