Protective effects of GTM-1 on endoplasmic reticulum stress induced by thapsgargin in rat neurons.
Protective effects of GTM-1 on endoplasmic reticulum stress induced by thapsgargin in rat neurons.
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GTM-1 对毒胡萝卜素诱导的大鼠神经元内质网应激的保护作用。
DOI：
10.1016/j.biopha.2016.09.050
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Peng Fu
中科院分区：
文献类型：
--
作者：
Lijuan Zhang;Lina Wang;Run Wang;Yonghua Pan;Yuan Gao;Peng Fu
GTM-1 is a drug that reverses Alzheimer’s Disease (AD) development specifically induced by thapsgargin (TG) and endoplasmic reticulum (ER) has been reported to be a pilot process that leads to AD formation. It is speculated that GTM-1 could also prohibit TG-induced ER stress. In this study, we utilized immuno-fluorescence to identify morphological changes in nucleus and transmission electron microscopy was used to observe neuronal ultra-structures. Moreover, expressions of GRP78, CHOP, Bcl-2 and cytochromecwere assessed using immuno-blotting, while calcium concentration was detected by fluorescence spectrometer. As suggested by the above cellular experiments, neuronal ultrastructures were damaged by the treatment of TG, while this damaging trend was reversed when neurons were simultaneously treated with both TG and GTM-1. Besides that, certain marker proteins of ER stress (e.g. GRP78, CHOP, and cytochromec) and calcium concentrations in neurons were significantly increased when TG was applied, while these levels were reduced to normal conditions when GTM-1 was added in the treatment. In conclusion, GTM-1 restrained the ongoing of ER stress that was induced by TG.
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影响因子：
2.9
作者：
V. Voccoli;F. Mazzoni;M. Garcia-Gil;L. Colombaioni
通讯作者：
V. Voccoli;F. Mazzoni;M. Garcia-Gil;L. Colombaioni
影响因子：
4
作者：
Quiroz-Baez, Ricardo;Ferrera, Patricia;Arias, Clorinda
通讯作者：
Arias, Clorinda
影响因子：
4.1
作者：
Gallego-Sandin, Sonia;Teresa Alonso, Maria;Garcia-Sancho, Javier
通讯作者：
Garcia-Sancho, Javier
影响因子：
7.2
作者：
Martinez, Juan A.;Zhang, Zhiqun;Larner, Stephen F.
通讯作者：
Larner, Stephen F.
影响因子：
4.8
作者：
Ding, Wen-Xing;Ni, Hong-Min;Yin, Xiao-Ming
通讯作者：
Yin, Xiao-Ming