Development of bFGF-Chitosan Matrices and Their Interactions with Human Dermal Fibroblast Cells
Development of bFGF-Chitosan Matrices and Their Interactions with Human Dermal Fibroblast Cells
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DOI：
10.1163/092050609x12457417534295
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发表时间：
2009-01-01
影响因子：
3.6
通讯作者：
Ghanem, Amyl
中科院分区：
文献类型：
--
作者：
Lefler, Amy;Ghanem, Amyl
壳聚糖（CH）是一种天然衍生的，可生物降解的葡萄糖胺聚合物，具有可变频率的N-乙酰基-D-葡萄糖胺单元，并已被证明具有许多药理学和伤口愈合特性。采用溶剂浇铸法制备了可生物降解的壳聚糖膜，并对其在皮肤组织工程中的应用进行了研究。碱性成纤维细胞生长因子（bFGF）纳入CH矩阵（1 μ g/膜）通过3种方法：吸附，包埋和共价结合。监测掺入的bFGF的释放速率和生物活性。使用人真皮成纤维细胞（HDF细胞）作为细胞对CH和bFGF-CH膜反应的体外模型。采用细胞附着、生长和酸溶性胶原定量作为细胞功能的评估。发现成纤维细胞在壳聚糖膜和支架上保持活力。不含bFGF的CH膜与HDF细胞相容;然而，成纤维细胞不增殖。从CH膜的吸附和结合的bFGF的释放曲线是相似的（表明结合是没有效率的），而截留的bFGF没有释放的时间框架研究。释放到细胞培养基中的bFGF浓度不足以刺激HDF增殖。然而，细胞附着显着增加，在壳聚糖膜与bFGF吸附到表面上相比，控制表面。在CH膜上生长的HDF细胞产生的胶原蛋白显著多于对照表面。(C)Koninklijke Brill NV，莱顿，2009年
Chitosan (CH) is a naturally derived, biodegradable polymer of glucosamine with a variable frequency of N-acetyl-D-glucosamine units, and has been demonstrated to have numerous pharmacological and wound-healing properties. Biodegradable chitosan films were fabricated using a solvent casting technique and investigated for skin tissue-engineering applications. Basic fibroblast growth factor (bFGF) was incorporated into the CH matrices (1 mu g/film) by 3 methods: adsorption, entrapment and covalent binding. Release rates and biological activity of the incorporated bFGF were monitored. Human dermal fibroblasts (HDF cells) were used as an in vitro model for cell response to CH and bFGF-CH films. Cell attachment, growth and acid-soluble collagen quantification were employed as an assessment of cell function. The fibroblasts were found to remain viable on the chitosan films and scaffolds. CH films without bFGF were compatible with HDF cells; however, the fibroblasts did not proliferate. The release profile of adsorbed and bound bFGF from CH films were similar (indicating that binding was not efficient) while entrapped bFGF was not released in the time frame studied. The concentration of bFGF released to the cell culture medium was not high enough to stimulate HDF proliferation. However, cell attachment was significantly increased in chitosan films with bFGF adsorbed onto the surface as compared to control surfaces. HDF cells grown on CH films produced significantly more collagen than those on control surfaces. (C) Koninklijke Brill NV, Leiden, 2009