T-cell antigen receptors with identical variable regions but different diversity and joining region gene segments have distinct specificities but cross-reactive idiotypes.
T-cell antigen receptors with identical variable regions but different diversity and joining region gene segments have distinct specificities but cross-reactive idiotypes.
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具有相同可变区但不同多样性和连接区基因片段的T细胞抗原受体具有不同的特异性，但具有交叉反应的独特型。
DOI：
10.1073/pnas.84.1.219
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发表时间：
1987
影响因子：
11.1
通讯作者：
Rolf H. Joho
中科院分区：
文献类型：
--
作者：
Fabio Rupp;Jolanda Brecher;Martin A. GIEDLINt;Tim MOSMANNt;R. Zinkernagel;H. Hengartner;Rolf H. Joho
描述了同种异体反应性H-2Db特异性细胞毒性T细胞克隆（3F 9）的T细胞抗原受体α链基因。这项研究和我们对3F 9 β链基因的研究表明，3F 9中表达的α和β链的可变区基因片段与鸡红细胞特异性I-Ab限制性辅助T细胞克隆（LB 2）所使用的基因片段相同。然而，这两个克隆的不同之处在于它们的T细胞受体分子的α和β链的多样性和连接部分。用3F 9 T细胞受体特异性单克隆抗体对3F 9和LB 2进行的分析表明，这两种T细胞克隆具有相同的独特型;然而，3F 9和LB 2未表现出任何抗原和/或主要组织相容性复合物交叉反应性。这表明T细胞受体的多样性和连接区域可能在抗原和/或主要组织相容性复合体识别中起关键作用。
The T-cell antigen receptor alpha-chain genes of an alloreactive, H-2Db-specific cytotoxic T-cell clone (3F9) are described. This study and our work on the 3F9 beta-chain genes reveal that the variable region gene segments for the alpha and beta chains expressed in 3F9 are identical to the ones used by a chicken erythrocyte-specific, I-Ab-restricted helper T-cell clone (LB2). These two clones differ, however, in the diversity and joining portions of the alpha and beta chains of their T-cell receptor molecules. The analysis of 3F9 and LB2 with monoclonal antibodies specific for the 3F9 T-cell receptor shows that these two T-cell clones share the same idiotype; however, 3F9 and LB2 do not exhibit any antigen and/or major histocompatibility complex cross-reactivity. This suggests that the diversity and joining regions of the T-cell receptor may play a key role in antigen and/or major histocompatibility complex recognition.