Early apoptosis in CD34+ cells as a potential heterogeneity in quality of cryopreserved umbilical cord blood
Early apoptosis in CD34+ cells as a potential heterogeneity in quality of cryopreserved umbilical cord blood
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DOI：
10.1111/j.1365-2141.2006.06270.x
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发表时间：
2006-10-01
影响因子：
6.5
通讯作者：
Oh, Il-Hoan
中科院分区：
文献类型：
--
作者：
Shim, Jae-Seung;Cho, Bin;Oh, Il-Hoan
脐带血(UCB)使用量的增加引发了有关冷冻保存的UCB质量的问题。这项研究探讨了CD34(+)细胞的早期凋亡是否是深低温保存的脐血功能异质性的一部分。CD34(+)PI(-)细胞的膜联蛋白V结合在新鲜和冷冻保存的UCBS中表现出很大的差异，在冷冻5年的单位之间差异更大。将分离的细胞异种移植到非肥胖糖尿病严重联合免疫缺陷小鼠中，结果表明，Annexin V检测确定了脐带血单位的大部分再繁殖活性。因此，CD34(+)细胞的早期凋亡可能会影响深低温保存的脐血移植的结果。
The increased use of umbilical cord blood (UCB) raises issues regarding the quality of cryopreserved UCB. This study investigated whether early apoptosis of CD34(+) cells is part of the functional heterogeneity of cryopreserved UCB. Annexin V binding of CD34(+)PI(-) cells showed wide variations in both fresh and cryopreserved UCBs, with greater variation among units frozen for > 5 years. Xenotransplantation of sorted cells into non-obese diabetic severe combined immunodeficient mice demonstrated that the Annexin V assay identified most repopulating activities in UCB units. Thus, early apoptosis of CD34(+) cells could influence the outcome of transplantation using cryopreserved UCB.