Alveolar microbiota profile in patients with human pulmonary tuberculosis and interstitial pneumonia
Alveolar microbiota profile in patients with human pulmonary tuberculosis and interstitial pneumonia
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DOI：
10.1016/j.micpath.2019.103851
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发表时间：
2020-02-01
影响因子：
3.8
通讯作者：
Teresa Herrera, Maria
中科院分区：
文献类型：
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作者：
Armando Vazquez-Perez, Joel;Ortega Carrillo, Concepcion;Teresa Herrera, Maria
背景：人类肺部微生物区系的存在已被证明存在于不同肺部疾病的患者中，主要是在痰标本中。然而，对于肺泡微生物区系的研究，支气管肺泡灌洗(BAL)样本代表下呼吸道(LRT)环境。目前尚不清楚肺结核、间质性肺炎等人类肺部疾病是否存在特定的肺泡微生物区系。方法：从6例活动性肺结核患者、6例间质性肺炎患者和10名健康志愿者的肺泡灌洗液中提取DNA，扩增细菌16S核糖体RNA基因(16S RDNA)。用MiSeq Desktop Sequencer测序仪对16S rDNA进行测序，并用QIIME软件进行分类鉴定。结果：TB和IP患者和健康志愿者的肺泡微生物区系由6个优势门组成，分别为革兰氏杆菌、变形杆菌、拟杆菌、放线菌、梭菌和蓝藻。在IP患者中观察到Firmicuts的丰度显著降低。在TB和IP患者中，肺泡微生物区系的多样性减少，其特征是链球菌属的丰度显著降低，并与TB患者的分枝杆菌丰度增加和IP患者的不动杆菌丰度相对于健康志愿者的丰度减少有关。然而，在结核病患者和IP患者之间观察到了一个重要的区别：结核病患者的梭菌丰度显著降低。结论：本研究表明，肺泡灌洗液标本中代表感染性和非感染性肺部疾病的肺泡微生物区系的多样性降低。
Background: The presence of the human lung microbiota has been demonstrated in patients with different lung diseases, mainly in sputum samples. However, for study of the alveolar microbiota, a bronchoalveolar lavage (BAL) sample represents the lower respiratory tract (LRT) environment. It is currently unknown whether there is a specific alveolar microbiota profile in human lung diseases, such as pulmonary tuberculosis (TB) and interstitial pneumonia (IP).Methods: BAL samples from six active TB patients, six IP patients and ten healthy volunteers were used for DNA extraction followed by amplification of the complete bacterial 16S ribosomal RNA gene (16S rDNA). The 16S rDNA was sequenced with a MiSeq Desktop Sequencer, and the data were analysed by QIIME software for taxonomic assignment.Results: The alveolar microbiota in TB and IP patients and healthy volunteers was characterized by six dominant phyla, Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Fusobacteria and Cyanobacteria. A significant reduction in the abundance of Firmicutes was observed in IP patients. In TB and IP patients, the diversity of the alveolar microbiota was diminished, characterized by a significant reduction in the abundance of the Streptococcus genus and associated with increased Mycobacterium abundance in TB patients and diminished Acinetobacter abundance in IP patients with respect to their abundances in healthy volunteers. However, an important difference was observed between TB and IP patients: the Fusobacterium abundance was significantly reduced in TB patients. Exclusive genera that were less abundant in patients than in healthy volunteers were characterized for each study group.Conclusions: This study shows that the alveolar microbiota profile in BAL samples from TB and IP patients, representing infectious and non-infectious lung diseases, respectively, is characterized by decreased diversity.