Maternal exposure of low dose of TCDD modulates the expression of estrogen receptor subunits of male gonads in offspring.
Maternal exposure of low dose of TCDD modulates the expression of estrogen receptor subunits of male gonads in offspring.
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DOI：
10.1292/jvms.69.619
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发表时间：
2007-06
期刊：
影响因子：
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通讯作者：
K. Ohyama;M. Ohta;T. Sano;Kazumasa Sato;Y. Nakagomi;Y. Shimura;Y. Yamano
中科院分区：
文献类型：
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作者：
K. Ohyama;M. Ohta;T. Sano;Kazumasa Sato;Y. Nakagomi;Y. Shimura;Y. Yamano
我们分析了低剂量经胎盘和哺乳期暴露于2,3,7,8-四氯二苯并-对二恶英（TCDD）对二恶英和性激素级联相关基因表达的影响，并证明了其对雄性后代大鼠睾丸生长和性成熟的影响。在妊娠第7、14天和分娩后第0、7、14天给药TCDD （10 ng/kg）。与对照组相比，17日龄大鼠肝脏细胞色素P450家族1亚家族A多肽1 （CYP1A1）基因表达显著升高。此外，与对照组相比，给药大鼠睾丸中雌激素受体(ER) α和erβ的表达在17日龄和42日龄时分别显著增加。虽然17日龄大鼠的睾丸重量和精小管直径增加，但与对照组相比，tcdd处理的生殖细胞数量没有差异。雄激素受体和抑制素亚基基因的表达无明显变化。这些发现表明，低剂量的TCDD暴露通过扰乱类固醇激素稳态导致睾丸异常发育。
We have analyzed the effects of low-dose transplacental and lactational exposure of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on gene expression relating to the dioxin and sexual hormone cascade, and demonstrated the effects on testicular growth and sexual maturation in male offspring rats. TCDD (10 ng/kg) was administered to dams on Days 7 and 14 of gestation, and on Days 0, 7 and 14 after delivery. Gene expression of cytochrome P450 family 1 subfamily A polypeptide 1 (CYP1A1) in the liver of 17-day-old rats was significantly increased compared with controls. Furthermore, expression of estrogen receptors (ER)alpha and ERbeta was significantly increased at 17 and 42 days old, respectively in the testis of TCDD-administered rats compared with controls. Although testicular weight and the seminiferous tubule diameter were increased in 17-day-old rats, there was no difference in the number of germ cells between TCDD-treated and control animals. The expressions of androgen receptor and inhibin subunit genes were not significantly changed. These findings suggest that low-dose exposure of TCDD leads to unusual development of the testis by perturbation of steroid hormone homeostasis.