Two functionally distinct classes of growth arrest states in human prokeratinocytes that regulate clonogenic potential.
Two functionally distinct classes of growth arrest states in human prokeratinocytes that regulate clonogenic potential.
复制标题
人类原角质形成细胞中两种功能不同的生长停滞状态，可调节克隆形成潜力。
DOI：
10.1111/1523-1747.ep12285684
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Scott,RE
中科院分区：
文献类型：
--
作者：
Pittelkow,MR;WilleJr,JJ;Scott,RE
在无血清培养基中培养的快速生长的正常人新生儿前角质形成细胞（HPK）可以被诱导在不同的细胞周期状态下经历可逆或不可逆的生长停滞。通过在人淋巴细胞条件培养基中培养低密度细胞、通过在高密度固定相条件培养基中培养以及通过在异亮氨酸缺陷培养基中培养诱导可逆的G1期阻滞。在生长因子缺乏的培养基中培养，HPK生长主要在G1期被不可逆地阻滞。在完全MCDB 153培养基中制备的甲基纤维素悬浮液中培养或在含血清培养基中培养也可诱导HPK在G1和G2期的不可逆生长停滞。最后，使用药物雷佐生诱导HPK在G2中的不可逆停滞。这些数据表明，有2种不同的HPK生长停滞状态，并建议每个逮捕机制可能在正常细胞的角质形成细胞分化的控制中发挥独特的作用。也有可能在这些过程中的任何一个选择性缺陷的发展可能是某些表皮疾病状态的病因学意义。
Rapidly growing normal human neonatal prokeratinocytes (HPK) cultured in serum-free medium can be induced to undergo either reversible or irreversible growth arrest at distinct cell cycle states. Reversible G1arrest was induced by culture of low-density cells in human lymphocyte conditioned medium, by culture in high-density stationary phase conditioned medium, and by culture in isoleucine-deficient medium. Irreversible arrest of HPK growth predominantly in G1was induced by culture in growth factor-deficient medium. Irreversible arrest of HPK growth in G1and G2was also induced by culture in suspension in methylcellulose prepared in complete MCDB 153 medium or by culture in serum-containing medium. Finally, the drug razoxane was employed to induce irreversible arrest of HPK in G2. These data establish that there are 2 distinct classes of growth arrest states for HPK and suggest that each arrest mechanism may serve a unique role in the control of keratinocyte differentiation in normal cells. It is also possible that the development of selective defects in either of these processes could be of etiologic significance in certain epidermal disease states.