Fluorescence microscopy investigations of the domain formation of fatty acid monolayers induced by polymeric gegenions
Fluorescence microscopy investigations of the domain formation of fatty acid monolayers induced by polymeric gegenions
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聚合gegenion诱导的脂肪酸单层结构域形成的荧光显微镜研究
DOI：
10.1021/la00058a058
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发表时间：
1991
期刊：
影响因子：
3.9
通讯作者：
H. Ringsdorf
中科院分区：
文献类型：
--
作者：
L. Chi;R. R. Johnston;H. Ringsdorf
自朗缪尔和亚当在空气/水界面上的不溶性单分子膜的开创性工作以来，二维长链脂肪酸的相态一直受到关注。1-3除了对纯水的研究外，许多研究还关注亚相条件（例如温度、pH或金属离子）对这些脂肪酸单层表面压力/面积等温线的影响。4-6单层的改变或在单层上实现特定效果的能力（例如，修补）具有很高的兴趣，特别是与它们作为生物膜模型的应用有关。7如果单分子层掺杂少量荧光探针，则可以通过荧光显微镜观察到脂在空气/水界面处的相态及其转变。8-10该技术允许在“两相”区域的聚集现象的可视化和在流体基质中的固体域的微观形态的研究。11-13此外，它允许研究单层的特殊条件（例如，杂质和添加剂14 -16）和亚相的特殊条件（例如，pH，电解质16 -17）对形式和性质的影响。
The phase states of long-chain fatty acids in two dimensions have been of interest since the pioneering work of Langmuir and Adam on insoluble monolayers at the air/water interface. 1-3 Besides the investigations on pure water, many studies havebeen concerned with the influence of subphase conditions, such as temperature, pH, or metal ions, on the surface pressure/area isotherms of these fatty acid monolayers. 4-6 Thealteration of mono-layers or the ability to achieve specific effects on mono-layers (eg, patching) is of high interestespecially in connection with their application as biomembrane models. 7 Phase states of lipids at the air/water interface and their transitions are observable via fluorescence micros-copy, if themonolayer is doped with small amounts of fluorescent probes. 8-10 This technique permits thevisualization of the aggregation phenomenon in the “two-phase” region and the study of the micromorphologies of solid domains in a fluid matrix. 11-13 Moreover, it allows one to study the influence of special conditions of the monolayer (eg, impurities and additives14-16) and of the subphase (eg, pH, electrolytes16-17) on the form and the