Rainbow trout gill cell line microplate cytotoxicity test
Rainbow trout gill cell line microplate cytotoxicity test
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DOI：
10.1007/1-4020-3120-3_16
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发表时间：
2005-01-01
期刊：
影响因子：
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通讯作者：
Bols, Niels C.
中科院分区：
文献类型：
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作者：
Dayeh, Vivian R.;Schirmer, Kristin;Bols, Niels C.
本章描述了一种用于评估水样毒性的快速、廉价的体外试验，以及在常规毒性试验中使用鱼类的潜在替代方法。该程序的总体目标是评估水样对鱼类细胞的急性或基础细胞毒性。基本细胞毒性是指所有或大多数细胞共有的细胞活动受损。基本程序也可用于评估单个化学品的急性细胞毒性，并了解其毒性背后的机制(S)。速度和节省成本的特点来自于评估96孔微孔板中鱼类细胞的生存能力。这降低了一次性用品的使用成本和将大量水样运往中央检测设施的成本。
This chapter describes a rapid, inexpensive in vitro test for evaluating the toxicity of water samples and a potential alternative to the use of fish in routine toxicity testing. The overall objective of the procedure is to assess water samples for acute or basal cytotoxicity to fish cells. Basal cytotoxicity refers to impairment of cellular activities that are shared by all or most cells. The basic procedure can also be used to evaluate the acute cytotoxicity of individual chemicals and to understand the mechanism (s) behind their toxicity. The speed and cost-saving features arise from assessing the viability of fish cells in 96-well microplates. This reduces the cost of disposables to be used and of shipping large volumes of water samples to a central testing facility.