Prophenoloxidase-Mediated Ex Vivo Immunity to Delay Fungal Infection after Insect Ecdysis.
Prophenoloxidase-Mediated Ex Vivo Immunity to Delay Fungal Infection after Insect Ecdysis.
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酚氧化酶原介导的离体免疫可延缓昆虫蜕皮后的真菌感染
DOI：
10.3389/fimmu.2017.01445
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发表时间：
2017
影响因子：
7.3
通讯作者：
Ling E
中科院分区：
文献类型：
--
作者：
Zhang J;Huang W;Yuan C;Lu Y;Yang B;Wang CY;Zhang P;Dobens L;Zou Z;Wang C;Ling E
Skin immunity protects animals from airborne pathogen infection. Unlike mammals, arthropods, including insects, undergo periodic ecdysis to grow and develop. Newly molted insects emerge with unsclerotized thin cuticles but successfully escape pathogenic infections during the post-molt period. Here we show that prophenoloxidases (PPOs) in molting fluids remain bioactive on the integument and impede fungal infection after ecdysis. We found that the purified plasma PPOs or recombinant PPOs could effectively bind to fungal spores (conidia) by targeting the cell wall components chitin and β-1,3-glucan. Pretreatment of the spores of the fungal pathogen Beauveria bassiana with PPOs increased spore hydrophilicity and reduced spore adhesion activity, resulting in a significant decrease in virulence as compared with mock infection. We also identified a spore-secreted protease BPS8, a member of peptidase S8 family of protease that degrade PPOs at high levels to benefit fungal infection, but which at lower doses activate PPOs to inhibit spore germination after melanization. These data indicate that insects have evolved a distinct strategy of ex vivo immunity to survive pathogen infections after ecdysis using PPOs in molting fluids retained on the underdeveloped and tender integument of newly molted insects for protection against airborne fungal infection.
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影响因子：
6.7
作者：
Binggeli O;Neyen C;Poidevin M;Lemaitre B
通讯作者：
Lemaitre B
影响因子：
4.8
作者：
Huang, Wei;Shang, Yanfang;Wang, Chengshu
通讯作者：
Wang, Chengshu
影响因子：
4.6
作者：
Lu HL;Wang JB;Brown MA;Euerle C;St Leger RJ
通讯作者：
St Leger RJ
影响因子：
5.4
作者：
Hu Y;Wang Y;Deng J;Jiang H
通讯作者：
Jiang H
果蝇先天免疫：酚氧化酶原的区域和功能专业化。
影响因子：
5.4
作者：
Dudzic JP;Kondo S;Ueda R;Bergman CM;Lemaitre B
通讯作者：
Lemaitre B