Spindlin, a major maternal transcript expressed in the mouse during the transition from oocyte to embryo.
Spindlin, a major maternal transcript expressed in the mouse during the transition from oocyte to embryo.
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DOI：
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发表时间：
1997-01
期刊：
影响因子：
4.6
通讯作者：
B. Oh;Sue‐Yun Hwang;D. Solter;B. Knowles
中科院分区：
文献类型：
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作者：
B. Oh;Sue‐Yun Hwang;D. Solter;B. Knowles
母体转录本的及时翻译及其基因产物的翻译后修饰控制着植入前胚胎的初始发育。我们已经分离并鉴定了一个编码特定阶段胚胎蛋白的基因。这种新的基因，Spindlin(Spin)，是一种丰富的母体转录本，存在于未受精卵和2-细胞阶段胚胎中，而不是8-细胞胚胎中。SPIN与一个多拷贝基因--Y-连锁精子发生特异性转录本(SSTY)有很高的同源性，它们共同形成了一个在配子发生过程中表达的新基因家族。我们发现Spindlin与减数分裂纺锤体相关，并以细胞周期依赖的方式被磷酸化修饰。此外，它迁移到前面描述的30x10(3)MR中期复合体，该复合体在第一个有丝分裂细胞周期中被翻译后修饰。我们的数据表明，纺锤体在配子到胚胎的转变过程中起着细胞周期调节的作用。
Timely translation of maternal transcripts and post-translational modification of their gene products control the initial development of preimplantation-stage embryos. We have isolated and characterized a gene encoding a stage-specific embryonic protein. This novel gene, spindlin (Spin), is an abundant maternal transcript present in the unfertilized egg and 2-cell, but not 8-cell, stage embryo. Spin exhibits high homology to a multicopy gene, Y-linked spermiogenesis-specific transcript (Ssty), and together they form a new gene family expressed during gametogenesis. We find that spindlin associates with the meiotic spindle and is modified by phosphorylation in a cell-cycle-dependent fashion. Furthermore, it comigrates with the previously described 30x10(3) Mr metaphase complex which is posttranslationally modified during the first mitotic cell cycle. Our data suggest that spindlin plays a role in cell-cycle regulation during the transition from gamete to embryo.