The characterization of gene expression during mouse neural stem cell differentiation in vitro
The characterization of gene expression during mouse neural stem cell differentiation in vitro
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DOI：
10.1016/j.neulet.2011.10.046
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发表时间：
2012-01
影响因子：
2.5
通讯作者：
J. H. Park;M. Choi;Kyoung Sun Park;S. H. Kim;K. Jung;Y. Chai
中科院分区：
文献类型：
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作者：
J. H. Park;M. Choi;Kyoung Sun Park;S. H. Kim;K. Jung;Y. Chai
神经干细胞（NSCs）是组织特异性的多能干细胞，在中枢神经系统中可以分化为三种细胞系：神经元、星形胶质细胞和少突胶质细胞。NSCs的治疗潜力推动了对调节其命运的基因表达特征的研究。本研究将胚胎第15天（E15）小鼠胚胎的NSCs分化1 （DF-1）或2 （DF-2）天，并通过微阵列和实时RT-PCR检测NSCs以及DF-1和DF-2细胞中的基因表达谱。在所分析的基因中，有1898个基因在DF-1和DF-2细胞中相对于NSCs表达上调，1642个基因表达下调。上调的基因包括Gfap、Smad6、Fst、Tgfb2和Cdkn2。下调的基因包括Ccnb1、Ccnd1和Ccnd2。我们使用匠心途径分析确定了与BMP和tgf - β 2信号通路相关的基因网络。我们的研究结果表明，E15 NSCs向星形胶质细胞的分化是基于多种信号通路的组合网络，包括细胞周期、BMP和tgf - β 2信号。
Neural stem cells (NSCs) are tissue-specific, multipotent stem cells that can differentiate into three cell lineages in the central nervous system: neurons, astrocytes and oligodendrocytes. The therapeutic potential of NSCs has fueled attempts to characterize the expression of genes that regulate their fate. In this study, NSCs from embryonic day 15 (E15) mouse embryos were differentiated for 1 (DF-1) or 2 (DF-2) days, and the gene expression patterns in the NSCs and in the DF-1 and DF-2 cells were measured by microarray and real-time RT-PCR. Among the analyzed genes, 1898 genes were up-regulated in the DF-1 and DF-2 cells relative to the NSCs, whereas 1642 genes were down-regulated. The up-regulated genes included Gfap, Smad6, Fst, Tgfb2 and Cdkn2. The down-regulated genes included Ccnb1, Ccnd1 and Ccnd2. We identified gene networks that were associated with BMP and TGF-beta2 signaling pathways using Ingenuity Pathway Analysis. Our results suggest that the differentiation of E15 NSCs into astrocytes is based on a combinatorial network of various signaling pathways, including cell cycle, BMP and TGF-beta2 signaling.