A Non-α-like DNA Polymerase from the Hyperthermophilic Archaeon Pyrococcus furiosus
A Non-α-like DNA Polymerase from the Hyperthermophilic Archaeon Pyrococcus furiosus
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来自超嗜热古菌激烈火球菌的非 α 样 DNA 聚合酶
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
Y. Ishino
中科院分区：
文献类型：
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作者：
M. Imamura;T. Uemori;I. Kato;Y. Ishino
我们确定了一种DNA聚合酶的性质，不同于那些α-样DNA聚合酶（Pol我），在激烈的热球菌，超嗜热古菌。新的DNA聚合酶（Pol II）进行了部分纯化和表征。Pol Ⅱ的DNA聚合活性对二脱氧胸苷三磷酸（ddTTP）较敏感，能被N-乙基马来酰亚胺抑制，而不能被阿非迪霉素抑制。活性染色凝胶电泳表明，DNA聚合活性来自于一个在十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳上分子量为130000的多肽。使用天然DNA模板的Pol II的DNA延伸能力远低于来自该生物体的Pol I，并且Pol II的DNA合成似乎是非进行性的。
We identified a DNA polymerase with properties that differed from those of alpha-like DNA polymerase (Pol I), in Pyrococcus furiosus, a hyperthermophilic archaeon. The novel DNA polymerase (Pol II) was partially purified and characterized. The DNA polymerizing activity of Pol II was relatively sensitive to dideoxythymidine-triphosphate (ddTTP) and it was inhibited by N-ethylmaleimide, but not by aphidicolin. Activity staining gel electrophoresis showed that the DNA polymerizing activity was derived from a polypeptide with a molecular weight of 130000 on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The DNA elongation ability of Pol II using a natural DNA template was much lower than that of Pol I from this organism and DNA synthesis of Pol II seems to be non-processive.