Mineralized matrix deposition by marrow stromal osteoblasts in 3D perfusion culture increases with increasing fluid shear forces
Mineralized matrix deposition by marrow stromal osteoblasts in 3D perfusion culture increases with increasing fluid shear forces
复制标题
DOI：
10.1073/pnas.2434367100
复制
发表时间：
2003-12-09
影响因子：
11.1
通讯作者：
Mikos, AG
中科院分区：
文献类型：
--
作者：
Sikavitsas, VI;Bancroft, GN;Mikos, AG
在这项研究中，我们报告了流体剪切应力对骨髓基质细胞成骨分化的直接参与。将大鼠骨髓基质细胞接种于三维多孔钛纤维网状支架中，在保持流体流速不变的情况下，在流式灌注生物反应器中灌注不同粘度的培养基，培养16 d。这种方法允许培养细胞以流体剪切应力的形式暴露于不断增加的机械刺激水平，而营养物质输送和废物清除的化学运输条件基本保持不变。3D多孔支架中培养的细胞在类似的化学转运下，流体剪切力的增加导致矿物质沉积的增加，这表明流体剪切力在3D流动灌注培养中提供的机械刺激确实可以增强成骨细胞表型的表达。流体剪切力的增加也导致三维钛纤维网状支架孔隙内部产生更好的空间分布的细胞外基质。流体剪切力对矿化细胞外基质产生和分布的综合影响强调了机械感觉对三维环境下成骨细胞功能的重要性。
In this study we report on direct involvement of fluid shear stresses on the osteoblastic differentiation of marrow stromal cells. Rat bone marrow stromal cells were seeded in 3D porous titanium fiber mesh scaffolds and cultured for 16 days in a flow perfusion bioreactor with perfusing culture media of different viscosities while maintaining the fluid flow rate constant. This methodology allowed exposure of the cultured cells to increasing levels of mechanical stimulation, in the form of fluid shear stress, whereas chemotransport conditions for nutrient delivery and waste removal remained essentially constant. Under similar chemotransport for the cultured cells in the 3D porous scaffolds, increasing fluid shear forces led to increased mineral deposition, suggesting that the mechanical stimulation provided by fluid shear forces in 3D flow perfusion culture can indeed enhance the expression of the osteoblastic phenotype. Increased fluid shear forces also resulted in the generation of a better spatially distributed extracellular matrix inside the porosity of the 3D titanium fiber mesh scaffolds. The combined effect of fluid shear forces on the mineralized extracellular matrix production and distribution emphasizes the importance of mechanosensation on osteoblastic cell function in a 3D environment.