Dietary Antigens Induce Germinal Center Responses in Peyer's Patches and Antigen-Specific IgA Production
Dietary Antigens Induce Germinal Center Responses in Peyer's Patches and Antigen-Specific IgA Production
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DOI：
10.3389/fimmu.2019.02432
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发表时间：
2019-10-15
影响因子：
7.3
通讯作者：
Ohno, Hiroshi
中科院分区：
文献类型：
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作者：
Hara, Satoko;Sasaki, Takaharu;Ohno, Hiroshi
肠道伊加的主要诱导部位是肠道相关淋巴组织（GALT），如派伊尔集合淋巴结（PP）和孤立淋巴滤泡（ILF）。已知肠道微生物群有助于肠道中伊加的产生;然而，对饮食抗原在伊加产生中的作用知之甚少。为了了解饮食抗原对伊加产生的影响，断奶后的小鼠维持在没有任何大的免疫原性分子的元素饮食上。我们发现饮食抗原通过诱导滤泡辅助性T细胞和生殖中心B细胞来促进PP中伊加的产生。通过在基础饲料中添加牛血清白蛋白（BSA）进一步证实了饲料抗原在PP反应中的作用。虽然饮食抗原对PP反应很重要，但它们对ILF的发育和成熟的影响比微生物群小。此外，我们证明，饮食抗原是必不可少的一个正常的抗原特异性伊加反应，伤寒沙门氏菌血清型鼠伤寒感染。这些结果提供了新的见解的作用，饮食抗原的粘膜免疫反应的调节。
The primary induction sites for intestinal IgA are the gut-associated lymphoid tissues (GALT), such as Peyer's patches (PPs) and isolated lymphoid follicles (ILFs). The commensal microbiota is known to contribute to IgA production in the gut; however, the role of dietary antigens in IgA production is poorly understood. To understand the effect of dietary antigens on IgA production, post-weaning mice were maintained on an elemental diet without any large immunogenic molecules. We found that dietary antigens contribute to IgA production in PPs through induction of follicular helper T cells and germinal center B cells. The role of dietary antigens in the PP responses was further confirmed by adding bovine serum albumin (BSA) into the elemental diet. Although dietary antigens are important for PP responses, they have fewer effects than the microbiota on the development and maturation of ILFs. Furthermore, we demonstrated that dietary antigens are essential for a normal antigen-specific IgA response to Salmonella typhi serovar Typhimurium infection. These results provide new insights into the role of dietary antigens in the regulation of mucosal immune responses.