The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function
The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function
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DOI：
10.1016/j.biomaterials.2010.09.008
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发表时间：
2011-01-01
期刊：
影响因子：
14
通讯作者：
Kwon, Yong-Dae
中科院分区：
文献类型：
--
作者：
Kim, Sung Eun;Song, Sang-Hun;Kwon, Yong-Dae
为了研究骨形态发生蛋白-2（rhBMP-2）在肝素接枝钛表面上的生物学功能，首先用3-氨丙基三乙氧基硅烷（ATPES）对钛表面进行表面修饰，然后在肝素接枝钛表面上接枝rhBMP-2。水接触角和蛋白质吸附的测量通过细胞增殖试验、碱性磷酸酶（ALP）活性的测量和钙沉积的测定来研究MG-63细胞在原始和功能化Ti表面上的生物活性。通过RT-PCR来评估抗炎作用，以测量IL-6和TNF-α的转录水平。α-XPS显示肝素和BMP-2分别成功地接枝和固定在钛表面上。此外，固定有BMP-2的钛表面比原始钛表面更亲水。当细胞生长在肝素接枝的BMP-2固定的Ti表面时，炎症反应也降低了。本研究的结果表明，通过接枝肝素和固定BMP-2，2在钛表面炎症可以抑制和成骨细胞功能促进（C）2010爱思唯尔有限公司版权所有
The aim of this study was to investigate biologic function of bone morphorgenic protein-2 (rhBMP-2) immobilized on the heparin-grafted Ti surface Ti surfaces were first modified by 3-amino-propyltriethoxysilane (ATPES) followed by grafting of heparin BMP-2 was then immobilized on the heparin-grafted Ti surfaces Pristine Ti and functionalized Ti surfaces were characterized by X-ray photoelectron spectroscopy (XPS) measurement of water contact angles and protein adsorption The biological activity of MG-63 cells on pristine and functionalized Ti surfaces was investigated by cell proliferation assays measurement of alkaline phosphate (ALP) activity and determination of calcium deposition Anti-inflammatory effects were assessed by RT-PCR to measure the transcript levels of IL-6 and TNF-alpha XPS revealed that heparin and BMP-2 were successfully grafted and immobilized on the Ti surfaces respectively In addition Ti surfaces with BMP-2 immobilized were more hydrophilic than pristine Ti Furthermore BMP-2 immobilized Ti promoted significantly higher ALP activity and calcium deposition by MG-63 cells than pristine Ti The inflammatory response was also decreased when cells were grown on heparin-grafted BMP-2-immobilized Ti surfaces The results of this study suggest that by grafting heparin and immobilizing BMP-2 on Ti surfaces inflammation can be inhibited and osteoblast function promoted (C) 2010 Elsevier Ltd All rights reserved