The hyp operon gene products are required for the maturation of catalytically active hydrogenase isoenzymes in Escherichia coli
The hyp operon gene products are required for the maturation of catalytically active hydrogenase isoenzymes in Escherichia coli
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大肠杆菌中催化活性氢化酶同工酶的成熟需要 hyp 操纵子基因产物
DOI：
10.1007/bf00276307
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发表时间：
2004
影响因子：
2.8
通讯作者：
A. Böck
中科院分区：
文献类型：
--
作者：
A. Jacobi;R. Rossmann;A. Böck
大肠杆菌的hyp操纵子包含合成所有三种氢化酶同工酶所需的几个基因。将删除引入到每个 hypA-E 基因中，转移到染色体上，并分析所得突变体的氢化酶 1、2 和 3 活性。三个基因 hypB、hypD 和 hypE 的产物被发现对于所有三种氢化酶同工酶的合成都是必需的。正如之前观察到的，hypB 的缺陷可以通过培养基中高浓度的镍来补充，而其他基因突变体的影响却不能。 hypA 的损伤阻止了氢化酶 3 活性的发展，不影响氢化酶 1 的水平，但导致氢化酶 2 活性显着增加，尽管氢化酶 2 蛋白的量没有显着改变。另一方面，hypC 的损伤导致氢化酶 1 活性降低并消除氢化酶 3 活性。因此，HYPA和HYPC除了是氢化酶3形成所必需的之外，还可能具有调节三种同工酶相对于彼此的活性并根据生理状况所施加的要求调节它们的水平的功能。所有五个 hyp 基因的突变都阻止了所有三种氢化酶同工酶大亚基的明显加工。结论是hypA-E基因的产物在镍掺入氢化酶脱辅基蛋白和/或该酶的组成亚基的加工中发挥作用。它们的作用的重要性也反映在它们在远缘相关生物中的系统发育保守性上。
The hyp operon of Escherichia coli comprises several genes which are required for the synthesis of all three hydrogenase isoenzymes. Deletions were introduced into each of the hypA-E genes, transferred to the chromosome and the resulting mutants were analysed for hydrogenase 1, 2 and 3 activity. The products of three of the genes, hypB, hypD and hypE were found to be essential for the synthesis of all three hydrogenase isoenzymes. A defect in hypB, as previously observed, could be complemented by high nickel concentrations in the medium, whereas the effects of mutants in the other genes could not. Lesions in hypA prevented development of hydrogenase 3 activity, did not influence the level of hydrogenase 1 but led to a considerable increase in hydrogenase 2 activity although the amount of hydrogenase 2 protein was not drastically altered. Lesions in hypC, on the other hand, led to a reduction of hydrogenase 1 activity and abolished hydrogenase 3 activity. HYPA and HYPC, besides being required for hydrogenase 3 formation, therefore may have a function in modulating the activities of the three isoenzymes with respect to each other and adjusting their levels to the requirement imposed by the physiological situation. Mutations in all five hyp genes prevented the apparent processing of the large subunits of all three hydrogenase isoenzymes. It is concluded that the products of the hypA-E genes play a role in nickel incorporation into hydrogenase apoprotein and/or processing of the constituent subunits of this enzyme. The importance of their roles is also reflected in their phylogenetic conservation in distantly related organisms.
影响因子：
11.3
作者：
A. Przybyla;Jeffery Robbins;N. Menon;H. D. Peck
通讯作者：
A. Przybyla;Jeffery Robbins;N. Menon;H. D. Peck