Arrestin can act as a regulator of rhodopsin photochemistry.
Arrestin can act as a regulator of rhodopsin photochemistry.
复制标题
Arrestin 可以充当视紫红质光化学的调节剂。
DOI：
10.1016/j.visres.2006.08.031
复制
发表时间：
2006
期刊：
影响因子：
1.8
通讯作者：
Farrens,DavidL
中科院分区：
文献类型：
--
作者：
Sommer,MarthaE;Farrens,DavidL
我们报道了视觉抑制蛋白可以调节视紫红质的视网膜释放和晚期光产物的形成。我们的实验，采用荧光标记的arrestin和视紫红质溶解在洗涤剂/磷脂胶束，表明arrestin可以捕获人口的视网膜在结合口袋中的吸光度特性的Meta II与完整的视网膜席夫碱。此外，抑制蛋白可以将Metarhodopsin III（通过热衰变或蓝光照射形成）转化为Meta II样吸收物质。总之，我们的结果表明，除了简单地淬灭转导蛋白活性之外，抑制蛋白可能还能够在视杆细胞中发挥更复杂的作用。这种可能性可能有助于解释为什么arrestin缺乏会导致静止性夜盲症（Oguchi病）和视网膜变性等问题。
We report that visual arrestin can regulate retinal release and late photoproduct formation in rhodopsin. Our experiments, which employ a fluorescently labeled arrestin and rhodopsin solubilized in detergent/phospholipid micelles, indicate that arrestin can trap a population of retinal in the binding pocket with an absorbance characteristic of Meta II with the retinal Schiff-base intact. Furthermore, arrestin can convert Metarhodopsin III (formed either by thermal decay or blue-light irradiation) to a Meta II-like absorbing species. Together, our results suggest arrestin may be able to play a more complex role in the rod cell besides simply quenching transducin activity. This possibility may help explain why arrestin deficiency leads to problems like stationary night blindness (Oguchi disease) and retinal degeneration.