Olea europaea (Oleaceae) phylogeography based on chloroplast DNA polymorphism
Olea europaea (Oleaceae) phylogeography based on chloroplast DNA polymorphism
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DOI：
10.1007/s00122-001-0832-x
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发表时间：
2002-06-01
影响因子：
5.4
通讯作者：
Bervillé, A
中科院分区：
文献类型：
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作者：
Besnard, G;Khadari, B;Bervillé, A
采用PCR-RFLP和微卫星标记技术对橄榄复合体叶绿体DNA多样性进行了研究。鉴定出15种绿型。我们构建了一个cpDNA系统发育树，其中五个进化枝被识别并位于不同的地理区域：进化枝a在非洲中部和南部，进化枝C在亚洲，进化枝M在非洲西北部，进化枝El在地中海盆地和撒哈拉，进化枝E2在西地中海。栽培橄榄簇拥地中海和撒哈拉野生形式（分支El和E2）。在地中海东部和西部橄榄之间观察到cpDNA标记的强烈遗传分化，表明这些地区代表了不同的冰川避难所。人类最有可能在地中海盆地西部传播一种东部叶绿体，在栽培品种中占优势。它在O. e.亚种中的存在。来自撒哈拉的laperrinei表明，地中海橄榄可能起源于非洲人群，这可能在第四纪期间在南地中海重叠。
Chloroplast DNA diversity in the olive (Olea europaea L.) complex was studied using PCR-RFLP and microsatellite markers. Fifteen chlorotypes were distinguished. We constructed a cpDNA phylogenetic tree in which five clades were recognised and located in distinct geographic areas: clade A in Central and Southern Africa, clade C in Asia, clade M in North-West Africa, clade El in the Mediterranean Basin and Sahara, and clade E2 in West Mediterranea. Cultivated olive clustered with Mediterranean and Saharan wild forms (clades El and E2). Strong genetic differentiation for cpDNA markers was observed between eastern and western Mediterranean olives, suggesting that these areas have represented different glacial refugia. Humans most likely spread one eastern chlorotype, preponderant in cultivars, across the western Mediterranean Basin. Its presence in O. e. subsp. laperrinei from the Sahara suggests a possible Mediterranean olive origin in an African population, which may have overlapped in the Southern Mediterranean during the Quaternary.