Proteomic and enzymatic response of poplar to cadmium stress
Proteomic and enzymatic response of poplar to cadmium stress
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DOI：
10.1016/j.jprot.2009.01.014
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发表时间：
2009-04-13
影响因子：
3.3
通讯作者：
Hausman, Jean-Francois
中科院分区：
文献类型：
--
作者：
Kieffer, Pol;Schroeder, Peter;Hausman, Jean-Francois
这项研究强调了镉胁迫暴露后生长旺盛的白杨植物根和叶的蛋白质组和酶的变化。在不同器官之间观察到响应于短期（14天）以及长期（56天）处理的蛋白质组学变化。在叶片中，热激蛋白、蛋白酶和病程相关蛋白等与胁迫相关的蛋白质含量增加。一种类似于植物-病原体相互作用的过敏反应似乎被诱导。就根而言，镉的代谢影响似乎比叶更有害。许多典型的应激相关蛋白如热休克蛋白或谷胱甘肽-S-转移酶的丰度早期增加，而来自初级代谢（糖酵解、三羧酸循环、氮代谢、硫代谢）的大多数蛋白质的丰度严重降低，证明了这一点。此外，镉对谷胱甘肽代谢的影响可以通过几种重要酶的活性测定来评估。镉处理对叶片中谷胱甘肽还原酶和抗坏血酸过氧化物酶有抑制作用，但在根中没有。相反，谷胱甘肽-S-转移酶表现出较高的活性（和丰度）在根中，但不是在叶中。(c)2009 Elsevier B. V.保留所有权利。
This study highlights proteomic and enzymatic changes in roots and leaves of actively growing poplar plants upon a cadmium stress exposure. Proteomic changes in response to a short-term (14 days), as well as a longer term (56 days) treatment are observed between the different organs. In leaves, stress-related proteins, like heat shock proteins, proteinases and pathogenesis-related proteins increased in abundance. A response similar to a hypersensitive response upon plant-pathogen interaction seemed to be induced. Concerning roots it appeared that the metabolic impact of cadmium was more deleterious than in leaves. This is evidenced by the early increase in abundance of many typical stress-related proteins like heat shock proteins, or glutathione-S-transferases, while most proteins from the primary metabolism (glycolysis, tricarboxylic acid cycle, nitrogen metabolism, sulfur metabolism) were severely decreased in abundance. Additionally the impact of cadmium on the glutathione metabolism could be assessed by activity assays of several important enzymes. Cadmium treatment had an inhibitory effect on glutathione reductase and ascorbate peroxidase in leaves, but not in roots. Conversely, glutathione-S-transferase showed a higher activity (and abundance) in roots but not in leaves. (c) 2009 Elsevier B.V. All rights reserved.