Chloroplast DNA diversity in Castanopsis hystrix populations in south China
Chloroplast DNA diversity in Castanopsis hystrix populations in south China
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DOI：
10.1016/j.foreco.2007.02.012
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发表时间：
2007-05
影响因子：
3.7
通讯作者：
Junyu Li;X. Ge;H. Cao;W. Ye
中科院分区：
文献类型：
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作者：
Junyu Li;X. Ge;H. Cao;W. Ye
对19个红锥中国居群的叶绿体DNA遗传多样性和遗传结构进行了量化分析。微卫星(SSR)用保守的引物进行聚合酶链式反应分析。测定群体内平均基因多样性(HS)、总基因多样性(HT)、无序等位基因分化(GST)和有序等位基因分化(NST)。检测到14种不同的单倍型，其中两种非常常见。居群间的分化水平(GST=23.6%)表明了一种高效的种子传播机制。此外，该种的GST和NST之间的差异不显著，表明该种的系统地理结构较弱或不存在。Hystrix单倍型的地理格局可以归因于它从众多而分散的避难所迁徙而来，该物种在最后一次冰川时期被限制在那里。这些结果为了解该树种的冰后重新定居模式提供了重要的洞察。
Nineteen Chinese populations of Castanopsis hystrix were examined to quantify genetic diversity and genetic structure at chloroplast DNA. Microsatellites (SSR) were analyzed by PCR using conserved primers. The average within population gene diversity (HS), the total gene diversity (HT), and the differentiation for unordered alleles (GST) and for ordered alleles (NST) were measured. Fourteen different haplotypes were detected, two of them very common. The level of differentiation among populations (GST=23.6%) indicates a highly efficient seed dispersal mechanism. In addition, the difference between GSTand NSTfor the species is not significant, suggesting that the phylogeographic structure is weak or absent. The geographical pattern of C. hystrix haplotypes could be attributed to its migration from the numerous and scattered refugia, where the species confined during the last glacial period. These results provide an important insight into patterns of postglacial recolonization of this tree species.